ArticleNature methods2008
Tracking transmitter-gated P2X cation channel activation in vitro and in vivo.
Article in Nature methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Energy Homeostasis Disruption in Neurological Disorders: Mitochondrial Dysfunction, High-Energy Phosphate Transfer, and Extracellular ATP-Dependent Purinergic Dysregulation.International journal of molecular sciences · 2026Review
- State of the art indicators for imaging purinergic dynamics in vitro and in vivo.Purinergic signalling · 2025Review
- Photoswitching alters fluorescence readout of jGCaMP8 CaProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Extracellular ATP/adenosine dynamics in the brain and its role in health and disease.Frontiers in cell and developmental biology · 2023Review
- New frontiers in probing the dynamics of purinergic transmitters in vivo.Neuroscience research · 2020Review
- Reducing Astrocyte Calcium Signaling In Vivo Alters Striatal Microcircuits and Causes Repetitive Behavior.Neuron · 2018Article
- Article
- Genetically Encoded Fluorescent Indicators for Organellar Calcium Imaging.Biophysical journal · 2016Review
- Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin.The Journal of general physiology · 2014Article
- Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway.Neuron · 2014Article
- Calcium-dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel.Nature cell biology · 2014Article
- Neuronal and glial purinergic receptors functions in neuron development and brain disease.Frontiers in cellular neuroscience · 2013Review
- Detection of endogenous substances with enzymatic microelectrode biosensors in the kidney.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Review
- Review
- Imaging P2X4 receptor lateral mobility in microglia: regulation by calcium and p38 MAPK.The Journal of biological chemistry · 2012Article
- P2X2 and P2X5 subunits define a new heteromeric receptor with P2X7-like properties.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Measurement of purine release with microelectrode biosensors.Purinergic signalling · 2012Article
- Neuronal P2X2 receptors are mobile ATP sensors that explore the plasma membrane when activated.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Time-lapse imaging of neural development: zebrafish lead the way into the fourth dimension.Genesis (New York, N.Y. : 2000) · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We present a noninvasive approach to track activation of ATP-gated P2X receptors and potentially other transmitter-gated cation channels that show calcium fluxes. We genetically engineered rat P2X receptors to carry calcium sensors near the channel pore and tested this as a reporter for P2X(2) receptor opening. The method has several advantages over previous attempts to image P2X channel activation by fluorescence resonance energy transfer (FRET): notably, it reports channel opening rather than a conformation change in the receptor protein. Our FRET-based imaging approach can be used as a general method to track, in real time, the location, regional expression variation, mobility and activation of transmitter-gated P2X channels in living neurons in vitro and in vivo. This approach should help to determine when, where and how different receptors are activated during physiological processes.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.