ArticleNature methods2008
A proteome chip approach reveals new DNA damage recognition activities in Escherichia coli.
Article in Nature methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed.
- Data storage and retrieval with unnatural proteins expressed via E. coli.Nature communications · 2026Article
- Editorial: Autoantibodies in cancer: diagnostic, prognostic, and therapeutic potential.Frontiers in oncology · 2026Article
- Anti-OLA1 autoantibody is a potential early diagnostic marker for hepatocellular carcinoma.Frontiers in immunology · 2025Article
- Reverse-Phase Protein Microarrays for OverexpressedAnalytical chemistry · 2024Article
- Diagnosis, Progress, and Treatment Update of Kawasaki Disease.International journal of molecular sciences · 2023Review
- Metabolic stress constrains microbial L-cysteine production in Escherichia coli by accelerating transposition through mobile genetic elements.Microbial cell factories · 2023Article
- Systematic Screening of Penetratin's Protein Targets by Yeast Proteome Microarrays.International journal of molecular sciences · 2022Article
- Multiple rather than specific autoantibodies were identified in irritable bowel syndrome with HuProt™ proteome microarray.Frontiers in physiology · 2022Article
- Using Omics to Study Leprosy, Tuberculosis, and Other Mycobacterial Diseases.Frontiers in cellular and infection microbiology · 2022Review
- Systematical Screening of Intracellular Protein Targets of Polyphemusin-I UsingInternational journal of molecular sciences · 2021Article
- Pediatric Tuberculosis: The Impact of "Omics" on Diagnostics Development.International journal of molecular sciences · 2020Review
- Developments and Applications of Functional Protein Microarrays.Molecular & cellular proteomics : MCP · 2020Review
- Identification of MltG as a Prc Protease Substrate Whose Dysregulation Contributes to the Conditional Growth Defect of Prc-DeficientFrontiers in microbiology · 2020Article
- Interplay between the bacterial protein deacetylase CobB and the second messenger c-di-GMP.The EMBO journal · 2019Article
- Structural Features of Transcription Factors Associating with Nucleosome Binding.Molecular cell · 2019Article
- A proteome-wide screen of Campylobacter jejuni using protein microarrays identifies novel and conformational antigens.PloS one · 2019Article
- Antibody Profiling of Kawasaki Disease UsingMolecular & cellular proteomics : MCP · 2018Article
- Global Analysis of SUMO-Binding Proteins Identifies SUMOylation as a Key Regulator of the INO80 Chromatin Remodeling Complex.Molecular & cellular proteomics : MCP · 2017Article
- Protein Array-based Approaches for Biomarker Discovery in Cancer.Genomics, proteomics & bioinformatics · 2017Review
- Escherichia coli Proteome Microarrays Identified the Substrates of ClpYQ Protease.Molecular & cellular proteomics : MCP · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Despite the fact that many genomes have been decoded, proteome chips comprising individually purified proteins have been reported only for budding yeast, mainly because of the complexity and difficulty of high-throughput protein purification. To facilitate proteomics studies in prokaryotes, we have developed a high-throughput protein purification protocol that allowed us to purify 4,256 proteins encoded by the Escherichia coli K12 strain within 10 h. The purified proteins were then spotted onto glass slides to create E. coli proteome chips. We used these chips to develop assays for identifying proteins involved in the recognition of potential base damage in DNA. By using a group of DNA probes, each containing a mismatched base pair or an abasic site, we found a small number of proteins that could recognize each type of probe with high affinity and specificity. We further evaluated two of these proteins, YbaZ and YbcN, by biochemical analyses. The assembly of libraries containing DNA probes with specific modifications and the availability of E. coli proteome chips have the potential to reveal important interactions between proteins and nucleic acids that are time-consuming and difficult to detect using other techniques.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.