ArticleNucleic acids research2008
In situ oligonucleotide synthesis on carbon materials: stable substrates for microarray fabrication.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates.Analytical chemistry · 2024Article
- Dipodal Silanes Greatly Stabilize Glass Surface Functionalization for DNA Microarray Synthesis and High-Throughput Biological Assays.Analytical chemistry · 2023Article
- Sequence-dependence of Cy3 and Cy5 dyes in 3' terminally-labeled single-stranded DNA.Scientific reports · 2022Article
- Simple synthesis of massively parallel RNA microarrays via enzymatic conversion from DNA microarrays.Nature communications · 2022Article
- Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA.RSC advances · 2022Article
- Molecular Surface Functionalization of Carbon Materials via Radical-Induced Grafting of Terminal Alkenes.Journal of the American Chemical Society · 2019Article
- Parallel DNA Synthesis on Poly(ethylene terephthalate).Chembiochem : a European journal of chemical biology · 2017Article
- Photolithographic Synthesis of High-Density DNA and RNA Arrays on Flexible, Transparent, and Easily Subdivided Plastic Substrates.Analytical chemistry · 2015Article
- Carbon Substrates: A Stable Foundation for Biomolecular Arrays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2015Review
- RNA-mediated gene assembly from DNA arrays.Angewandte Chemie (International ed. in English) · 2012Article
- In situ Synthesis of Oligonucleotide Arrays on Surfaces Coated with Crosslinked Polymer Multilayers.Chemistry of materials : a publication of the American Chemical Society · 2012Article
- Efficiency, error and yield in light-directed maskless synthesis of DNA microarrays.Journal of nanobiotechnology · 2011Article
- Rapid determination of RNA accessible sites by surface plasmon resonance detection of hybridization to DNA arrays.Analytical chemistry · 2009Article
- Photopatterned thiol surfaces for biomolecule immobilization.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Fabrication and characterization of DNA arrays prepared on carbon-on-metal substrates.Analytical chemistry · 2009Article
- Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.Sensors and actuators. B, Chemical · 2009Article
- Controlling oligonucleotide surface density in light-directed DNA array fabrication.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Acyl chloride-modified amorphous carbon substrates for the attachment of alcohol-, thiol-, and amine-containing molecules.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Attaching molecules to chlorinated and brominated amorphous carbon substrates via Grignard reactions.Langmuir : the ACS journal of surfaces and colloids · 2009Article
- Aldehyde-terminated amorphous carbon substrates for the fabrication of biomolecule arrays.Langmuir : the ACS journal of surfaces and colloids · 2008Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Glass has become the standard substrate for the preparation of DNA arrays. Typically, glass is modified using silane chemistries to provide an appropriate functional group for nucleic acid synthesis or oligonucleotide immobilization. We have found substantial issues with the stability of these surfaces as manifested in the unwanted release of oligomers from the surface when incubated in aqueous buffers at moderate temperatures. To address this issue, we have explored the use of carbon-based substrates. Here, we demonstrate in situ synthesis of oligonucleotide probes on carbon-based substrates using light-directed photolithographic phosphoramidite chemistry and evaluate the stabilities of the resultant DNA arrays compared to those fabricated on silanized glass slides. DNA arrays on carbon-based substrates are substantially more stable than arrays prepared on glass. This superior stability enables the use of high-density DNA arrays for applications involving high temperatures, basic conditions, or where serial hybridization and dehybridization is desired.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.