ArticleNucleic acids research2008
Analysis of intermolecular base pair formation of prohead RNA of the phage phi29 DNA packaging motor using NMR spectroscopy.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- DNA/RNA hybrids avoid channel gating that leads to the continued packaging of numerous hybrids into the phi29 protein shell.Nucleic acids research · 2025Article
- An unexpected RNA distal interaction mode found in an essential region of the hepatitis C virus genome.Nucleic acids research · 2018Article
- Prohead RNA: a noncoding viral RNA of novel structure and function.Wiley interdisciplinary reviews. RNA · 2016Review
- Structural features of a 3' splice site in influenza a.Biochemistry · 2015Article
- Insights into the structure and assembly of the bacteriophage 29 double-stranded DNA packaging motor.Journal of virology · 2014Article
- Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells.Nature protocols · 2013Article
- Crystal structure of 3WJ core revealing divalent ion-promoted thermostability and assembly of the Phi29 hexameric motor pRNA.RNA (New York, N.Y.) · 2013Article
- Structure of the RNA claw of the DNA packaging motor of bacteriophage Φ29.Nucleic acids research · 2012Article
- Structure and assembly of the essential RNA ring component of a viral DNA packaging motor.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Different sequences show similar quaternary interaction stabilities in prohead viral RNA self-assembly.The Journal of biological chemistry · 2011Article
- The emerging field of RNA nanotechnology.Nature nanotechnology · 2010Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The bacteriophage ø29 DNA packaging motor that assembles on the precursor capsid (prohead) contains an essential 174-nt structural RNA (pRNA) that forms multimers. To determine the structural features of the CE- and D-loops believed to be involved in multimerization of pRNA, 35- and 19-nt RNA molecules containing the CE-loop or the D-loop, respectively, were produced and shown to form a heterodimer in a Mg2+-dependent manner, similar to that with full-length pRNA. It has been hypothesized that four intermolecular base pairs are formed between pRNA molecules. Our NMR study of the heterodimer, for the first time, proved directly the existence of two intermolecular Watson-Crick G-C base pairs. The two potential intermolecular A-U base pairs were not observed. In addition, flexibility of the D-loop was found to be important since a Watson-Crick base pair introduced at the base of the D-loop disrupted the formation of the intermolecular G-C hydrogen bonds, and therefore affected heterodimerization. Introduction of this mutation into the biologically active 120-nt pRNA (U80C mutant) resulted in no detectable dimerization at ambient temperature as shown by native gel and sedimentation velocity analyses. Interestingly, this pRNA bound to prohead and packaged DNA as well as the wild-type 120-nt pRNA.
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Registered trials
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