ArticleApplied and environmental microbiology2008
Differentiation of Clostridium botulinum serotype A strains by multiple-locus variable-number tandem-repeat analysis.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Why Are Botulinum Neurotoxin-Producing Bacteria So Diverse and Botulinum Neurotoxins So Toxic?Toxins · 2019Review
- Genetic Diversity of Clostridium sporogenes PA 3679 Isolates Obtained from Different Sources as Resolved by Pulsed-Field Gel Electrophoresis and High-Throughput Sequencing.Applied and environmental microbiology · 2016Article
- Clostridium botulinum Group II Isolate Phylogenomic Profiling Using Whole-Genome Sequence Data.Applied and environmental microbiology · 2015Article
- Historical and current perspectives on Clostridium botulinum diversity.Research in microbiology · 2015Review
- Genetic characterization and comparison of Clostridium botulinum isolates from botulism cases in Japan between 2006 and 2011.Applied and environmental microbiology · 2014Article
- Distinguishing highly-related outbreak-associated Clostridium botulinum type A(B) strains.BMC microbiology · 2014Article
- Mass Spectrometric Identification and Differentiation of Botulinum Neurotoxins through Toxin Proteomics.Reviews in analytical chemistry · 2013Article
- Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes.Applied and environmental microbiology · 2012Article
- Subtyping botulinum neurotoxins by sequential multiple endoproteases in-gel digestion coupled with mass spectrometry.Analytical chemistry · 2012Article
- De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics.Analytical and bioanalytical chemistry · 2012Article
- Exploring genomic diversity inThe botulinum journal · 2012Article
- Clostridium botulinum group I strain genotyping by 15-locus multilocus variable-number tandem-repeat analysis.Journal of clinical microbiology · 2011Article
- Analysis of Clostridium botulinum serotype E strains by using multilocus sequence typing, amplified fragment length polymorphism, variable-number tandem-repeat analysis, and botulinum neurotoxin gene sequencing.Applied and environmental microbiology · 2011Article
- Comparison of PCR ribotyping and multilocus variable-number tandem-repeat analysis (MLVA) for improved detection of Clostridium difficile.BMC microbiology · 2011Article
- Independent evolution of neurotoxin and flagellar genetic loci in proteolytic Clostridium botulinum.BMC genomics · 2009Article
- Genetic homogeneity of Clostridium botulinum type A1 strains with unique toxin gene clusters.Applied and environmental microbiology · 2008Article
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ten variable-number tandem-repeat (VNTR) regions identified within the complete genomic sequence of Clostridium botulinum strain ATCC 3502 were used to characterize 59 C. botulinum strains of the botulism neurotoxin A1 (BoNT/A1) to BoNT/A4 (BoNT/A1-A4) subtypes to determine their ability to discriminate among the serotype A strains. Two strains representing each of the C. botulinum serotypes B to G, including five bivalent strains, and two strains of the closely related species Clostridium sporogenes were also tested. Amplified fragment length polymorphism analyses revealed the genetic diversity among the serotypes and the high degree of similarity among many of the BoNT/A1 strains. The 10 VNTR markers amplified fragments within all of the serotype A strains but were less successful with strains of other serotypes. The composite multiple-locus VNTR analysis of the 59 BoNT/A1-A4 strains and 3 bivalent B strains identified 38 different genotypes. Thirty genotypes were identified among the 53 BoNT/A1 and BoNT/A1(B) strains, demonstrating discrimination below the subtype level. Contaminating DNA within crude toxin preparations of three BoNT/A subtypes (BoNT/A1 to BoNT/A3) also supported amplification of all of the VNTR regions. These markers provide clinical and forensics laboratories with a rapid, highly discriminatory tool to distinguish among C. botulinum BoNT/A1 strains for investigations of botulism outbreaks.
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