ArticleApplied and environmental microbiology2008
The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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77 citing papers in PubMed, 153 citations in OpenAlex.
- The Lrs14-Like AbfR1 Homolog From Metallosphaera sedula Is a Nucleoid-Organizing Protein.MicrobiologyOpen · 2025Article
- Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition.Journal of biological engineering · 2025Article
- The Lrs14 family of DNA-binding proteins as nucleoid-associated proteins in the Crenarchaeal order Sulfolobales.Molecular microbiology · 2025Review
- Article
- Chalcopyrite bioleaching efficacy by extremely thermoacidophilic archaea leverages balanced iron and sulfur biooxidation.Bioresource technology · 2024Article
- Development of a defined medium for the heterotrophic cultivation of Metallosphaera sedula.Extremophiles : life under extreme conditions · 2024Article
- Complete genome sequence for the thermoacidophilic archaeonMicrobiology resource announcements · 2024Article
- Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials.Frontiers in microbiology · 2024Article
- Interplay between transcriptional regulators and VapBC toxin-antitoxin loci during thermal stress response in extremely thermoacidophilic archaea.Environmental microbiology · 2023Article
- Enhancement Mechanism of Stibnite Dissolution Mediated byInternational journal of molecular sciences · 2022Article
- Fox Cluster determinants for iron biooxidation in the extremely thermoacidophilic Sulfolobaceae.Environmental microbiology · 2022Article
- Thermoacidophilic Bioleaching of Industrial Metallic Steel Waste Product.Frontiers in microbiology · 2022Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Life in hot acid: a genome-based reassessment of the archaeal order Sulfolobales.Environmental microbiology · 2021Article
- Archaea: An Agro-Ecological Perspective.Current microbiology · 2021Review
- Diatoms: Miniscule biological entities with immense importance in synthesis of targeted novel bioparticles and biomonitoring.Journal of biosciences · 2021Review
- Physiology, Taxonomy, and Sulfur Metabolism of the Sulfolobales, an Order of Thermoacidophilic Archaea.Frontiers in microbiology · 2021Review
- The Proposed Molecular Mechanisms Used by Archaea for Fe(III) Reduction and Fe(II) Oxidation.Frontiers in microbiology · 2021Review
- Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis.Nature chemical biology · 2020Review
- Comparative Genomic Analysis Reveals the Metabolism and Evolution of the Thermophilic Archaeal GenusFrontiers in microbiology · 2020Article
17 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Despite their taxonomic description, not all members of the order Sulfolobales are capable of oxidizing reduced sulfur species, which, in addition to iron oxidation, is a desirable trait of biomining microorganisms. However, the complete genome sequence of the extremely thermoacidophilic archaeon Metallosphaera sedula DSM 5348 (2.2 Mb, approximately 2,300 open reading frames [ORFs]) provides insights into biologically catalyzed metal sulfide oxidation. Comparative genomics was used to identify pathways and proteins involved (directly or indirectly) with bioleaching. As expected, the M. sedula genome contains genes related to autotrophic carbon fixation, metal tolerance, and adhesion. Also, terminal oxidase cluster organization indicates the presence of hybrid quinol-cytochrome oxidase complexes. Comparisons with the mesophilic biomining bacterium Acidithiobacillus ferrooxidans ATCC 23270 indicate that the M. sedula genome encodes at least one putative rusticyanin, involved in iron oxidation, and a putative tetrathionate hydrolase, implicated in sulfur oxidation. The fox gene cluster, involved in iron oxidation in the thermoacidophilic archaeon Sulfolobus metallicus, was also identified. These iron- and sulfur-oxidizing components are missing from genomes of nonleaching members of the Sulfolobales, such as Sulfolobus solfataricus P2 and Sulfolobus acidocaldarius DSM 639. Whole-genome transcriptional response analysis showed that 88 ORFs were up-regulated twofold or more in M. sedula upon addition of ferrous sulfate to yeast extract-based medium; these included genes for components of terminal oxidase clusters predicted to be involved with iron oxidation, as well as genes predicted to be involved with sulfur metabolism. Many hypothetical proteins were also differentially transcribed, indicating that aspects of the iron and sulfur metabolism of M. sedula remain to be identified and characterized.
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