ArticleApplied and environmental microbiology2008
Anaerobic conditions promote expression of Sfp fimbriae and adherence of sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM to human intestinal epithelial cells.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
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- Use of synthetic communities to study microbial ecology of the gut.Microbiome research reports · 2022Review
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- Pathogenomes of Atypical Non-shigatoxigenicFrontiers in microbiology · 2020Article
- Origin and Evolution of Hybrid Shiga Toxin-Producing and Uropathogenic Escherichia coli Strains of Sequence Type 141.Journal of clinical microbiology · 2019Article
- Genomic Analysis of Putative Virulence Factors Affecting Cytotoxicity ofFrontiers in microbiology · 2019Article
- Modulation of EnterohaemorrhagicMicroorganisms · 2018Review
- Food-Associated Stress Primes Foodborne Pathogens for the Gastrointestinal Phase of Infection.Frontiers in microbiology · 2018Review
- Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:HApplied and environmental microbiology · 2017Article
- Article
- A Small Regulatory RNA Contributes to the Preferential Colonization ofFrontiers in microbiology · 2017Article
- Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path.Applied and environmental microbiology · 2016Article
- Shiga toxin production and translocation during microaerobic human colonic infection with Shiga toxin-producing E. coli O157:H7 and O104:H4.Cellular microbiology · 2014Article
- Recent advances in understanding enteric pathogenic Escherichia coli.Clinical microbiology reviews · 2013Review
- Growth media simulating ileal and colonic environments affect the intracellular proteome and carbon fluxes of enterohemorrhagic Escherichia coli O157:H7 strain EDL933.Applied and environmental microbiology · 2013Article
- Molecular mechanisms that mediate colonization of Shiga toxin-producing Escherichia coli strains.Infection and immunity · 2012Review
- Enhanced Actin Pedestal Formation by Enterohemorrhagic Escherichia coli O157:H7 Adapted to the Mammalian Host.Frontiers in microbiology · 2011Article
- Microaerobic conditions enhance type III secretion and adherence of enterohaemorrhagic Escherichia coli to polarized human intestinal epithelial cells.Environmental microbiology · 2010Article
- Comparative analysis of attachment of Shiga-toxigenic Escherichia coli and Salmonella strains to cultured HT-29 and Caco-2 cell lines.Applied and environmental microbiology · 2009Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sfp gene cluster, unique to sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:NM strains, encodes fimbriae that mediate mannose-resistant hemagglutination in laboratory E. coli strains but are not expressed in wild-type SF EHEC O157:NM strains under standard laboratory conditions. We investigated whether Sfp fimbriae are expressed under conditions that mimic the intestinal environment and whether they contribute to the adherence of SF EHEC O157:NM strains to human intestinal epithelial cells. The transcription of sfpA (encoding the major fimbrial subunit) was upregulated in all strains investigated, and all expressed SfpA and possessed fimbriae that reacted with an anti-SfpA antibody when the strains were grown on solid media under anaerobic conditions. Sfp expression was absent under aerobic conditions and in liquid media. Sfp upregulation under anaerobic conditions was significantly higher on blood agar and a medium simulating the colonic environment than on a medium simulating the ileal environment (P < 0.05). The induction of Sfp fimbriae in SF E. coli O157:NM strains correlates with increased adherence to Caco-2 and HCT-8 cells. Our data indicate that the expression of Sfp fimbriae in SF E. coli O157:NM strains is induced under conditions resembling those of the natural site of infection and that Sfp fimbriae may contribute to the adherence of the organisms to human intestinal epithelium.
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