ArticleThe Journal of biological chemistry2008
EP2 and EP4 receptors regulate aromatase expression in human adipocytes and breast cancer cells. Evidence of a BRCA1 and p300 exchange.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed, 89 citations in OpenAlex.
- Obesity and endocrine therapy resistance in breast cancer: Mechanistic insights and perspectives.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2022Review
- Regulation of Estradiol Synthesis by Aromatase Interacting Partner in Breast (AIPB).Molecular and cellular biology · 2021Article
- Functional Genetic Variation in the Anti-Müllerian Hormone Pathway in Women With Polycystic Ovary Syndrome.The Journal of clinical endocrinology and metabolism · 2019Article
- Prostaglandin EThe Journal of biological chemistry · 2019Article
- Understanding the tumour micro-environment communication network from an NOS2/COX2 perspective.British journal of pharmacology · 2019Review
- The Impact of Obesity on Breast Cancer.Current oncology reports · 2018Review
- Regulation of inflammation by lipid mediators in oral diseases.Oral diseases · 2017Review
- Metabolic Obesity, Adipose Inflammation and Elevated Breast Aromatase in Women with Normal Body Mass Index.Cancer prevention research (Philadelphia, Pa.) · 2017Article
- Hsp90 and PKM2 Drive the Expression of Aromatase in Li-Fraumeni Syndrome Breast Adipose Stromal Cells.The Journal of biological chemistry · 2016Article
- Role of EP2 and EP4 receptors in airway microvascular leak induced by prostaglandin E2.British journal of pharmacology · 2016Article
- Obesity and Cancer: Concepts and Challenges.Indian journal of surgical oncology · 2015Article
- Estrogen Protects against Obesity-Induced Mammary Gland Inflammation in Mice.Cancer prevention research (Philadelphia, Pa.) · 2015Article
- Genetics of androgen metabolism in women with infertility and hypoandrogenism.Nature reviews. Endocrinology · 2015Review
- PKA signaling drives mammary tumorigenesis through Src.Oncogene · 2015Article
- Prostaglandin E2 (EP) receptors mediate PGE2-specific events in ovulation and luteinization within primate ovarian follicles.Endocrinology · 2014Article
- EP2 signaling mediates suppressive effects of celecoxib on androgen receptor expression and cell proliferation in prostate cancer.Prostate cancer and prostatic diseases · 2014Article
- Prostaglandin E receptor EP4 is a therapeutic target in breast cancer cells with stem-like properties.Breast cancer research and treatment · 2014Article
- Estrogen as Jekyll and Hyde: regulation of cell death.F1000Research · 2014Article
- PPARγ activation inhibits growth and survival of human endometriotic cells by suppressing estrogen biosynthesis and PGE2 signaling.Endocrinology · 2013Article
- Molecular pathways: adipose inflammation as a mediator of obesity-associated cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2013Article
Corrections and comments
- Retraction · 2019-12-02Duplication of/in Image · · Dannenberg retired March 2021 - http://medicine.weill.cornell.edu/sites/default/files/wnim_2021-03.pdf
- Commented on by
- Commented on by
- RetractedWithdrawal: EP2020
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Cytochrome P450 aromatase (aromatase), a product of the CYP19 gene, catalyzes the synthesis of estrogens from androgens. Because aromatase-dependent estrogen biosynthesis has been linked to hormone-dependent breast carcinogenesis, it is important to elucidate the mechanisms that regulate CYP19 gene expression. The main objective of this study was to identify the receptors (EP) for prostaglandin E(2) (PGE(2)) that mediate the induction of CYP19 transcription in human adipocytes and breast cancer cells. Treatment with PGE(2) induced aromatase, an effect that was mimicked by either EP(2) or EP(4) agonists. Antagonists of EP(2) or EP(4) or small interference RNA-mediated down-regulation of these receptors suppressed PGE(2)-mediated induction of aromatase. PGE(2) via EP(2) and EP(4) stimulated the cAMP-->protein kinase A pathway resulting in enhanced interaction between P-CREB, p300, and the aromatase promoter I.3/II. Overexpressing a mutant form of p300 that lacks histone acetyltransferase activity suppressed PGE(2)-mediated induction of aromatase promoter activity. PGE(2) via EP(2) and EP(4) also caused a reduction in both the amounts of BRCA1 and the interaction between BRCA1 and the aromatase promoter I.3/II. Activation of the aromatase promoter by PGE(2) was suppressed by overexpressing wild-type BRCA1. Silencing of EP(2) or EP(4) also blocked PGE(2)-mediated induction of the progesterone receptor, a prototypic estrogen-response gene. In a mouse model, overexpressing COX-2 in the mammary gland, a known inducer of PGE(2) synthesis, led to increased aromatase mRNA and activity and reduced amounts of BRCA1; these effects were reversed by knocking out EP(2). Taken together, these results suggest that PGE(2) via EP(2) and EP(4) activates the cAMP-->PKA-->CREB pathway leading to enhanced CYP19 transcription and increased aromatase activity. Reciprocal changes in the interaction between BRCA1, p300, and the aromatase promoter I.3/II contributed to the inductive effects of PGE(2).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.