ArticleThe Journal of biological chemistry2008
Human myosin Vc is a low duty ratio nonprocessive motor.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Motor properties of Myosin 5c are modulated by tropomyosin isoforms and inhibited by pentabromopseudilin.Frontiers in physiology · 2024Article
- Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells.International journal of molecular sciences · 2022Article
- Small teams of myosin Vc motors coordinate their stepping for efficient cargo transport on actin bundles.The Journal of biological chemistry · 2017Article
- Myosin Vc Is Specialized for Transport on a Secretory Superhighway.Current biology : CB · 2016Article
- Identification of the Isoform-specific Interactions between the Tail and the Head of Class V Myosin.The Journal of biological chemistry · 2016Article
- The Role of the Cytoskeleton and Myosin-Vc in the Targeting of KCa3.1 to the Basolateral Membrane of Polarized Epithelial Cells.Frontiers in physiology · 2016Article
- Myosin motor isoforms direct specification of actomyosin function by tropomyosins.Cytoskeleton (Hoboken, N.J.) · 2015Article
- The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis.The Journal of cell biology · 2014Article
- Coupling of two non-processive myosin 5c dimers enables processive stepping along actin filaments.Scientific reports · 2014Article
- Structural insights into the assembly of a monomeric class V myosin.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Fission yeast tropomyosin specifies directed transport of myosin-V along actin cables.Molecular biology of the cell · 2014Article
- Multiple roles for the actin cytoskeleton during regulated exocytosis.Cellular and molecular life sciences : CMLS · 2013Review
- Tropomyosin is essential for processive movement of a class V myosin from budding yeast.Current biology : CB · 2012Article
- Plant-Specific Myosin XI, a Molecular Perspective.Frontiers in plant science · 2012Article
- Walking to work: roles for class V myosins as cargo transporters.Nature reviews. Molecular cell biology · 2011Review
- Shaking the myosin family tree: biochemical kinetics defines four types of myosin motor.Seminars in cell & developmental biology · 2011Review
- Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex.The Journal of cell biology · 2011Article
- A nonprocessive class V myosin drives cargo processively when a kinesin- related protein is a passenger.Current biology : CB · 2009Article
- Myosin Vc is a molecular motor that functions in secretory granule trafficking.Molecular biology of the cell · 2009Article
- Essential features of the class V myosin from budding yeast for ASH1 mRNA transport.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
There are three distinct members of the myosin V family in vertebrates, and each isoform is involved in different membrane trafficking pathways. Both myosin Va and Vb have demonstrated that they are high duty ratio motors that are consistent with the processive nature of these motors. Here we report that the ATPase cycle mechanism of the single-headed construct of myosin Vc is quite different from those of other vertebrate myosin V isoforms. K(ATPase) of the actin-activated ATPase was 62 microm, which is much higher than that of myosin Va ( approximately 1 mum). The rate of ADP release from actomyosin Vc was 12.7 s(-1), which was 2 times greater than the entire ATPase cycle rate, 6.5 s(-1). P(i) burst size was 0.31, indicating that the equilibrium of the ATP hydrolysis step is shifted to the prehydrolysis form. Our kinetic model, based on all kinetic data we determined in this study, suggests that myosin Vc spends the majority of the ATPase cycle time in the weak actin binding state in contrast to myosin Va and Vb. Consistently, the two-headed myosin Vc construct did not show processive movement in total internal reflection fluorescence microscope analysis, demonstrating that myosin Vc is a nonprocessive motor. Our findings suggest that myosin Vc fulfills its function as a cargo transporter by different mechanisms from other myosin V isoforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.