ArticleJournal of the American Chemical Society2008
Pyrene excimer signaling molecular beacons for probing nucleic acids.
Article in Journal of the American Chemical Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Generating, visualizing and tuning π-π interactions in metal-induced supramolecular assembly.CrystEngComm · 2026Article
- Internal Dynamics of Pyrene-Labeled Polyols Studied Through the Lens of Pyrene Excimer Formation.Polymers · 2025Article
- Nanoscale covalent organic frameworks for enhanced photocatalytic hydrogen production.Nature communications · 2024Article
- Sculpting photoproducts with DNA origami.Chem · 2024Article
- Time-resolved Fluorescence DNA-based Sensors for Reducing Background Fluorescence of Environment.Journal of fluorescence · 2023Review
- High-Throughput Analysis of Bacterial Toxic Lipopolysaccharide in Water by Dual-Wavelength Monitoring Using a Ratiometric Fluorescent Chemosensor.Analytical chemistry · 2023Article
- Recent Advances in Excimer-Based Fluorescence Probes for Biological Applications.Molecules (Basel, Switzerland) · 2022Review
- Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2.Sensors and actuators. B, Chemical · 2022Article
- Three's a crowd - stabilisation, structure, and applications of DNA triplexes.Chemical science · 2022Review
- Conjugation of chemical handles and functional moieties to DNA during solid phase synthesis with sulfonyl azides.Nucleic acids research · 2022Article
- Research progress of live-cell RNA imaging techniques.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022Review
- Design of Large Stokes Shift Fluorescent Proteins Based on Excited State Proton Transfer of an Engineered Photobase.Journal of the American Chemical Society · 2021Article
- Computer visionRSC advances · 2021Article
- Electrosynthesis and Electrochromism of a New Crosslinked Polydithienylpyrrole with Diphenylpyrenylamine Subunits.Polymers · 2020Article
- Pyrene Excimer-Based Fluorescent Labeling of Cysteines Brought into Close Proximity by Protein Dynamics: ASEM-Induced Thiol-Ene Click Reaction for High Spatial Resolution CLEM.International journal of molecular sciences · 2020Article
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- Interaction of Cucurbit[7]uril With Protease Substrates: Application to Nanosecond Time-Resolved Fluorescence Assays.Frontiers in chemistry · 2020Article
- Hydrophilic, Red-Emitting, and Thermally Activated Delayed Fluorescence Emitter for Time-Resolved Luminescence Imaging by Mitochondrion-Induced Aggregation in Living Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Fluorescence Based Investigation of Temperature-Dependent PbJournal of fluorescence · 2019Article
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Authors and funding
11 authors.
Funding
Abstract
Molecular beacon DNA probes, containing 1-4 pyrene monomers on the 5' end and the quencher DABCYL on the 3' end, were engineered and employed for real-time probing of DNA sequences. In the absence of a target sequence, the multiple-pyrene labeled molecular beacons (MBs) assumed a stem-closed conformation resulting in quenching of the pyrene excimer fluorescence. In the presence of target, the beacons switched to a stem-open conformation, which separated the pyrene label from the quencher molecule and generated an excimer emission signal proportional to the target concentration. Steady-state fluorescence assays resulted in a subnanomolar limit of detection in buffer, whereas time-resolved signaling enabled low-nanomolar target detection in cell-growth media. It was found that the excimer emission intensity could be scaled by increasing the number of pyrene monomers conjugated to the 5' terminal. Each additional pyrene monomer resulted in substantial increases in the excimer emission intensities, quantum yields, and excited-state lifetimes of the hybridized MBs. The long fluorescence lifetime ( approximately 40 ns), large Stokes shift (130 nm), and tunable intensity of the excimer make this multiple-pyrene moiety a useful alternative to traditional fluorophore labeling in nucleic acid probes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.