ArticleJournal of virology2008
Antiretroviral activity of ancestral TRIM5alpha.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Matryoshka Dolls of Virus Evolution: The Internal Forces That Shape Viral Fate.Integrative and comparative biology · 2025Article
- Cyclophilin A Regulates Tripartite Motif 5 Alpha Restriction of HIV-1.International journal of molecular sciences · 2025Review
- Not so unique to Primates: The independent adaptive evolution of TRIM5 in Lagomorpha lineage.PloS one · 2019Article
- Robustness of Reconstructed Ancestral Protein Functions to Statistical Uncertainty.Molecular biology and evolution · 2017Article
- Expression levels of Fv1: effects on retroviral restriction specificities.Retrovirology · 2016Article
- Article
- Trans-species polymorphism in humans and the great apes is generally maintained by balancing selection that modulates the host immune response.Human genomics · 2015Review
- Lentiviral Protein Transfer Vectors Are an Efficient Vaccine Platform and Induce a Strong Antigen-Specific Cytotoxic T Cell Response.Journal of virology · 2015Article
- Evolutionary and Functional Analysis of Old World Primate TRIM5 Reveals the Ancient Emergence of Primate Lentiviruses and Convergent Evolution Targeting a Conserved Capsid Interface.PLoS pathogens · 2015Article
- African green monkey TRIM5α restriction in simian immunodeficiency virus-specific rhesus macaque effector CD4 T cells enhances their survival and antiviral function.Journal of virology · 2015Article
- Potent restriction of HIV-1 and SIVmac239 replication by African green monkey TRIM5α.Retrovirology · 2015Article
- Evolution of the retroviral restriction gene Fv1: inhibition of non-MLV retroviruses.PLoS pathogens · 2014Article
- A cross-species view on viruses.Current opinion in virology · 2012Review
- Studies of endogenous retroviruses reveal a continuing evolutionary saga.Nature reviews. Microbiology · 2012Review
- Host genes important to HIV replication and evolution.Cold Spring Harbor perspectives in medicine · 2012Review
- Paleovirology - ghosts and gifts of viruses past.Current opinion in virology · 2011Review
- Unique spectrum of activity of prosimian TRIM5alpha against exogenous and endogenous retroviruses.Journal of virology · 2011Article
- Homology-based identification of capsid determinants that protect HIV1 from human TRIM5α restriction.The Journal of biological chemistry · 2011Article
- Generation of human TRIM5alpha mutants with high HIV-1 restriction activity.Gene therapy · 2010Article
- Paleovirology--modern consequences of ancient viruses.PLoS biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The antiretroviral protein TRIM5alpha is known to have evolved different restriction capacities against various retroviruses, driven by positive Darwinian selection. However, how these different specificities have evolved in the primate lineages is not fully understood. Here we used ancestral protein resurrection to estimate the evolution of antiviral restriction specificities of TRIM5alpha on the primate lineage leading to humans. We used TRIM5alpha coding sequences from 24 primates for the reconstruction of ancestral TRIM5alpha sequences using maximum-likelihood and Bayesian approaches. Ancestral sequences were transduced into HeLa and CRFK cells. Stable cell lines were generated and used to test restriction of a panel of extant retroviruses (human immunodeficiency virus type 1 [HIV-1] and HIV-2, simian immunodeficiency virus [SIV] variants SIV(mac) and SIV(agm), and murine leukemia virus [MLV] variants N-MLV and B-MLV). The resurrected TRIM5alpha variant from the common ancestor of Old World primates (Old World monkeys and apes, approximately 25 million years before present) was effective against present day HIV-1. In contrast to the HIV-1 restriction pattern, we show that the restriction efficacy against other retroviruses, such as a murine oncoretrovirus (N-MLV), is higher for more recent resurrected hominoid variants. Ancestral TRIM5alpha variants have generally limited efficacy against HIV-2, SIV(agm), and SIV(mac). Our study sheds new light on the evolution of the intrinsic antiviral defense machinery and illustrates the utility of functional evolutionary reconstruction for characterizing recently emerged protein differences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.