ArticleJournal of clinical microbiology2008
Early infant human immunodeficiency virus type 1 detection suitable for resource-limited settings with multiple circulating subtypes by use of nested three-monoplex DNA PCR and dried blood spots.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- A Rapid and Sensitive Detection of HIV-1 with a One-Pot Two-Stage Reverse Transcription Recombinase Aided Real-Time PCR Assay.Tropical medicine and infectious disease · 2023Article
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Adjusting for time-dependent sensitivity in an illness-death model, with application to mother-to-child transmission of HIV.Statistics in medicine · 2015Article
- Determinant and outcome of early diagnosis of HIV infection among HIV-exposed infants in southwest Ethiopia.BMC research notes · 2014Article
- Past, present and future molecular diagnosis and characterization of human immunodeficiency virus infections.Emerging microbes & infections · 2012Review
- Diagnosis of HIV-1 infection in infants using dried blood spots in Tamil Nadu, South India.Indian journal of sexually transmitted diseases and AIDS · 2011Article
- Quality assurance in the HIV/AIDS laboratory network of China.International journal of epidemiology · 2010Article
- Clinical evaluation of a dried blood spot assay for atazanavir.Antimicrobial agents and chemotherapy · 2010Article
- Nucleic acid assay system for tier II laboratories and moderately complex clinics to detect HIV in low-resource settings.The Journal of infectious diseases · 2010Article
- Detection of low levels of human immunodeficiency virus (HIV) may be critical for early diagnosis of pediatric HIV infection by use of dried blood spots.Journal of clinical microbiology · 2009Article
- Article
- Update on successes and challenges regarding mother-to-child transmission of HIV.Current opinion in pediatrics · 2009Review
- Qualitative human immunodeficiency virus RNA analysis of dried blood spots for diagnosis of infections in infants.Journal of clinical microbiology · 2009Article
- Overseas processing of dried blood spots for timely diagnosis of HIV in Haitian infants.Revista panamericana de salud publica = Pan American journal of public health · 2008Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The early detection of human immunodeficiency virus type 1 (HIV-1) infection in infants is complicated by the persistence of maternal antibodies and by diverse HIV-1 subtypes. We developed a nested, three-monoplex HIV-1 DNA PCR (N3M-PCR) assay to detect diverse HIV-1 subtypes in infants born to infected mothers. We optimized the test for use with dried blood spot (DBS) samples for ease of storage and transport from rural China to central laboratories. Six pairs of primers were designed that targeted env, gag, and pol genes, and the test was run in three reactions with an analytical sensitivity of 10 copies DNA per reaction to cover nine HIV-1 subtypes, A, B, C, D, F, G, CRF01-AE, CRF08-BC, and CRF07-BC. The assay performance was evaluated on 347 DBS specimens from 151 exposed infants in four diverse provinces of China in which multiple subtypes were circulating. The results of this test were compared to those of HIV antibody enzyme immunoassay and Western blotting confirmation for the infants at > or =18 months of age or to convincing clinical and epidemiologic data for deceased infants. The sensitivity of the N3M-PCR assay was 30.0% (3/10) for infants at 48 h after birth, 91.7% (11/12) at 1 to 2 months of age, and 93.7% (15/16) at 3 to 6 months of age. The specificity was 100% (94/94) at all three time points. The PCR reproducibility in the three DNA regions was 100% for samples at 48 h after birth, 96.7% at 1 to 2 months, and 100% at 3 to 6 months of age. The HIV-1 DNA N3M-PCR assay on DBSs offers a simple and affordable approach for early infant HIV-1 diagnosis in regions with diverse HIV-1 circulating subtypes.
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