ArticleClinical and vaccine immunology : CVI2008
VirB12 is a serological marker of Brucella infection in experimental and natural hosts.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 34 citations in OpenAlex.
- Differentiating infected from vaccinated animals: Validation and application of NH-AGID assay in heifers immunized with Brucella abortus strain A19-∆VirB12.Veterinary and animal science · 2025Article
- Design and characterization of a high-resolution multiple-SNP capture array by target sequencing for sheep.Journal of animal science · 2023Article
- Evaluation and Differential Diagnosis of a Genetic Marked Brucella Vaccine A19ΔvirB12 for Cattle.Frontiers in immunology · 2021Article
- Brucella melitensis VirB12 recombinant protein is a potential marker for serodiagnosis of human brucellosis.Annals of clinical microbiology and antimicrobials · 2017Article
- Mechanism and Function of Type IV Secretion During Infection of the Human Host.Microbiology spectrum · 2016Review
- Investigating the use of protein saver cards for storage and subsequent detection of bovine anti-Brucella abortus smooth lipopolysaccharide antibodies and gamma interferon.Clinical and vaccine immunology : CVI · 2013Article
- Bacterial Type IV secretion systems: versatile virulence machines.Future microbiology · 2012Review
- Systems biology approach predicts antibody signature associated with Brucella melitensis infection in humans.Journal of proteome research · 2011Article
- Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens.PLoS neglected tropical diseases · 2010Article
- Brucella regulators: self-control in a hostile environment.Trends in microbiology · 2009Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The Brucella species type IV secretion system, encoded by the virB1-12 locus, is required for intracellular replication and persistent infection in vivo. The requirement of VirB proteins for infection suggests that they are expressed in vivo and may therefore represent serological markers of infection. To test this idea, we purified recombinant VirB1, VirB5, VirB11, and VirB12 and tested for their recognition by antibodies in sera from experimentally infected mice and goats by using an indirect enzyme-linked immunosorbent assay. Antibody responses to VirB12 but not to VirB1, VirB5, or VirB11 were detected in 20/20 mice experimentally inoculated with Brucella abortus and 12/12 goats experimentally infected with Brucella melitensis. The potential use of VirB12 as a serological tool for the diagnosis of brucellosis was evaluated in the natural bovine host. Serum samples from 145 cattle of known serology (29% negative and 71% positive) were analyzed for the production of antibody responses to VirB12. One hundred two cattle samples (70.3%) were positive for antibodies to VirB12, while 43 samples were negative (29.7%). A positive serological response to VirB12 correlated with positive serology to whole B. abortus antigen in 99% of samples tested. These results show that VirB12 is expressed during infection of both experimental and natural hosts of Brucella species, and they suggest that VirB12 may be a useful serodiagnostic marker for brucellosis.
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