ArticleClinical and vaccine immunology : CVI2008
Detection of Mycobacterium avium subsp. paratuberculosis by a sonicate immunoassay based on surface-enhanced Raman scattering.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 85 citations in OpenAlex.
- Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Faeces Using a Rhodamine B Hydrazone Immunosensor on Antibody-Coated Nitrocellulose Membranes.Veterinary medicine and science · 2026Article
- Comparison of Immunomagnetic Bead Separation-Immunosensor Detection and Nested-PCR Methods for Detecting Mycobacterium avium Subspecies paratuberculosis in Cattle Feces.Journal of clinical laboratory analysis · 2025Article
- Review
- Immunoassays: Analytical and Clinical Performance, Challenges, and Perspectives of SERS Detection in Comparison with Fluorescent Spectroscopic Detection.International journal of molecular sciences · 2024Review
- Introducing a portable electrochemical biosensor for Mycobacterium avium subsp. paratuberculosis detection using graphene oxide and chitosan.Scientific reports · 2024Article
- Saliva NIR spectroscopy and Aquaphotomics: a novel diagnostic approach to Paratuberculosis in dairy cattle.Frontiers in cellular and infection microbiology · 2024Article
- Aluminum Foil vs. Gold Film: Cost-Effective Substrate in Sandwich SERS Immunoassays of Biomarkers Reveals Potential for Selectivity Improvement.International journal of molecular sciences · 2023Article
- Dual-enhancement and dual-tag design for SERS-based sandwich immunoassays: evaluation of a metal-metal effect in 3D architecture.Mikrochimica acta · 2021Article
- Nanotechnologies in Food Science: Applications, Recent Trends, and Future Perspectives.Nano-micro letters · 2020Review
- SERS detection ofAnalytica chimica acta: X · 2019Article
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Detection and Characterization of Antibiotic-Resistant Bacteria Using Surface-Enhanced Raman Spectroscopy.Nanomaterials (Basel, Switzerland) · 2018Review
- Surface-Enhanced Raman Scattering-Based Immunoassay Technologies for Detection of Disease Biomarkers.Biosensors · 2017Review
- Nanomaterial-based sensors for detection of foodborne bacterial pathogens and toxins as well as pork adulteration in meat products.Journal of food and drug analysis · 2016Review
- Host Transcriptional Profiles and Immunopathologic Response following Mycobacterium avium subsp. paratuberculosis Infection in Mice.PloS one · 2015Article
- Succinimidyl ester surface chemistry: implications of the competition between aminolysis and hydrolysis on covalent protein immobilization.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- Regulatory T Cell Activity and Signs of T Cell Unresponsiveness in Bovine Paratuberculosis.Frontiers in veterinary science · 2014Article
- Toward development of a surface-enhanced Raman scattering (SERS)-based cancer diagnostic immunoassay panel.The Analyst · 2013Article
- Recent progress in SERS biosensing.Physical chemistry chemical physics : PCCP · 2011Review
- Detection of the potential pancreatic cancer marker MUC4 in serum using surface-enhanced Raman scattering.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sandwich immunoassay for the rapid, low-level detection of Mycobacterium avium subsp. paratuberculosis has been developed. M. avium subsp. paratuberculosis is the causative agent of Johne's disease in cattle, and one of the major obstacles in controlling the spread of this disease is the inability to rapidly detect small amounts of bacteria or other diagnostic markers shed during the subclinical stage of infection. This paper details the development and performance of an assay for sonicated M. avium subsp. paratuberculosis lysate that is based on surface-enhanced Raman scattering (SERS). There are two key components of the assay: (i) an immobilized layer of monoclonal antibodies that target a surface protein on the microorganism; and (ii) extrinsic Raman labels (ERLs) that are designed to selectively bind to captured proteins and produce large SERS signals. By correlating the number of M. avium subsp. paratuberculosis bacilli present prior to sonication to the amount of total protein in the resulting sonicate, the detection limit determined for total protein can be translated to the microorganism concentration. These findings yield detection limits of 100 and 200 ng/ml (estimated to be 500 and 1,000 M. avium subsp. paratuberculosis bacilli/ml) for sonicate spiked in phosphate buffer and sonicate spiked in whole milk, respectively. Moreover, the time required to complete the assay, which includes sample preparation, antigen extraction, ERL incubation, and readout, is less than 24 h. The potential for incorporation of this novel assay into diagnostic laboratories is also briefly discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.