ArticleProceedings of the National Academy of Sciences of the United States of America2007
A high-affinity molybdate transporter in eukaryotes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 141 citations in OpenAlex.
- Homeostasis of Molybdenum, Xanthine Oxidase and Aldehyde Oxidase Activity Levels in the Livers of Patients with End-Stage Liver Failure and Healthy Controls.International journal of molecular sciences · 2026Article
- Biological use of molybdenum and tungsten stems back to 3.4 billion years ago.Nature communications · 2026Article
- Relationship and distribution patterns between geochemical backgrounds of W and MScientific reports · 2026Article
- Natural Variation in the Promoter of OsMOT1;1 Contributes to Differential Molybdenum Accumulation between Indica and Japonica Rice.Rice (New York, N.Y.) · 2025Article
- Transcriptomic analysis reveals the role of the molybdate transporter2 in mediating nitrate stress tolerance in tomato seedlings.BMC plant biology · 2025Article
- OsDISMO1: A Novel Transporter for Molybdenum Distribution in Rice Shoots.Rice (New York, N.Y.) · 2025Article
- Insertional Mutagenesis as a Strategy to Open New Paths in Microalgal Molybdenum and Nitrate Homeostasis.Current issues in molecular biology · 2025Article
- Elemental profiling and genome-wide association studies reveal genomic variants modulating ionomic composition inFrontiers in plant science · 2024Article
- The Mechanisms of Molybdate Distribution and Homeostasis with Special Focus on the Model PlantMolecules (Basel, Switzerland) · 2023Review
- Moonlighting Arabidopsis molybdate transporter 2 family and GSH-complex formation facilitate molybdenum homeostasis.Communications biology · 2023Article
- Review
- Precise Quantification of Molybdate In Vitro by the FRET-Based Nanosensor 'MolyProbe'.Molecules (Basel, Switzerland) · 2022Article
- Physiological Importance of Molybdate Transporter Family 1 in Feeding the Molybdenum Cofactor Biosynthesis Pathway inMolecules (Basel, Switzerland) · 2022Article
- Transcriptomic, Metabolomic and Ionomic Analyses Reveal Early Modulation of Leaf Mineral Content inInternational journal of molecular sciences · 2022Article
- The Vacuolar Molybdate Transporter OsMOT1;2 Controls Molybdenum Remobilization in Rice.Frontiers in plant science · 2022Article
- Genome-Wide Association Study Reveals Genomic Regions Associated With Molybdenum Accumulation in Wheat Grains.Frontiers in plant science · 2022Article
- Engineering of molybdenum-cofactor-dependent nitrate assimilation in Yarrowia lipolytica.FEMS yeast research · 2021Article
- Genetic architecture of root and shoot ionomes in rice (Oryza sativa L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Molybdate toxicity in Chinese cabbage is not the direct consequence of changes in sulphur metabolism.Plant biology (Stuttgart, Germany) · 2020Article
- Integrated Analysis of Molybdenum Nutrition and Nitrate Metabolism in Strawberry.Frontiers in plant science · 2020Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molybdenum is an essential element for almost all living beings, which, in the form of a molybdopterin-cofactor, participates in the active site of enzymes involved in key reactions of carbon, nitrogen, and sulfur metabolism. This metal is taken up by cells in form of the oxyanion molybdate. Bacteria acquire molybdate by an ATP-binding-cassette (ABC) transport system in a widely studied process, but how eukaryotic cells take up molybdenum is unknown because molybdate transporters have not been identified so far. Here, we report a eukaryotic high-affinity molybdate transporter, encoded by the green alga Chlamydomonas reinhardtii gene MoT1. An antisense RNA strategy over the MoT1 gene showed that interference of the expression of this gene leads to the inhibition of molybdate transport activity and, in turn, of the Mo-containing enzyme nitrate reductase, indicating a function of MoT1 in molybdate transport. MOT1 functionality was also shown by heterologous expression in Saccharomyces cerevisiae. Molybdate uptake mediated by MOT1 showed a K(m) of approximately 6 nM, which is the range of the lowest K(m) values reported and was activated in the presence of nitrate. Analysis of deduced sequence from the putative protein coded by MoT1 showed motifs specifically conserved in similar proteins present in the databases, and defines a family of membrane proteins in both eukaryotes and prokaryotes probably involved in molybdate transport and distantly related to plant sulfate transporters SULTR. These findings represent an important step in the understanding of molybdate transport, a crucial process in eukaryotic cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.