ArticleProceedings of the National Academy of Sciences of the United States of America2007
Sequence of late molecular events in the activation of rhodopsin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 134 citations in OpenAlex.
- Activation of visual rhodopsin probed by single-shot transient IR spectroscopy.Biophysical journal · 2026Article
- Hidden water's influence on rhodopsin activation.Biophysical journal · 2024Review
- Review
- Article
- Structural aspects of rod opsin and their implication in genetic diseases.Pflugers Archiv : European journal of physiology · 2021Review
- DEER Analysis of GPCR Conformational Heterogeneity.Biomolecules · 2021Review
- Capturing Peptide-GPCR Interactions and Their Dynamics.Molecules (Basel, Switzerland) · 2020Review
- A Conserved Proline Hinge Mediates Helix Dynamics and Activation of Rhodopsin.Structure (London, England : 1993) · 2020Article
- G protein-coupled receptors of class A harness the energy of membrane potential to increase their sensitivity and selectivity.Biochimica et biophysica acta. Biomembranes · 2019Article
- Stepwise activation of a class C GPCR begins with millisecond dimer rearrangement.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Crystal structure of rhodopsin in complex with a mini-GScience advances · 2018Article
- The arrestin-1 finger loop interacts with two distinct conformations of active rhodopsin.The Journal of biological chemistry · 2018Article
- Protein Sequence and Membrane Lipid Roles in the Activation Kinetics of Bovine and Human Rhodopsins.Biophysical journal · 2017Article
- Conformational equilibria of light-activated rhodopsin in nanodiscs.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Retinal orientation and interactions in rhodopsin reveal a two-stage trigger mechanism for activation.Nature communications · 2016Article
- The Molecular Switching Mechanism at the Conserved D(E)RY Motif in Class-A GPCRs.Biophysical journal · 2016Article
- Beyond spectral tuning: human cone visual pigments adopt different transient conformations for chromophore regeneration.Cellular and molecular life sciences : CMLS · 2016Article
- CONDENSED-MATTER SPECTROSCOPY SPECTRAL METHODS FOR STUDY OF THE G-PROTEIN-COUPLED RECEPTOR RHODOPSIN. II. MAGNETIC RESONANCE METHODS.Optics and spectroscopy · 2016Article
- A Usual G-Protein-Coupled Receptor in Unusual Membranes.Angewandte Chemie (International ed. in English) · 2016Article
- Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Activation of the G protein-coupled receptor rhodopsin involves both the motion of transmembrane helix 6 (TM6) and proton exchange events. To study how these activation steps relate to each other, spin-labeled rhodopsin in solutions of dodecyl maltoside was used so that time-resolved TM6 motion and proton exchange could each be monitored as a function of pH and temperature after an activating light flash. The results reveal that the motion of TM6 is not synchronized with deprotonation of the Schiff base that binds the chromophore to the protein but is an order of magnitude slower at 30 degrees C. However, TM6 motion and the uptake of a proton from solution in the neutral pH range follow the same time course. Importantly, the motion of TM6 is virtually independent of pH, as is Schiff base deprotonation under the conditions used, whereas proton uptake titrates with a pK of 6.5. This finding shows that proton uptake is a consequence rather than a cause of helix motion. Activated rhodopsin binds to and subsequently activates the cognate G protein, transducin. It has been shown that peptides derived from the C terminus of the transducin alpha-subunit mimic in part binding of the intact G protein. These peptides are found to bind to rhodopsin after TM6 movement, resulting in the release of protons. Collectively, the data suggest the following temporal sequence of events involved in activation: (i) internal Schiff base proton transfer; (ii) TM6 movement; and (iii) proton uptake from solution and binding of transducin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.