ArticlePlant cell reports2008
Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 115 citations in OpenAlex.
- From scorching sands to survival: adaptive tale of the genus Tribulus (T. longipetalus, T. terrestris and T. pentandrus) in desert ecosystem.Die Naturwissenschaften · 2025Article
- Isolation and HPLC assisted quantification of two iridoid glycoside compounds and molecular DNA fingerprinting in critically endangered medicinalPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Amplified Fragment Length Polymorphism: Applications and Recent Developments.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Molecular characterization ofSaudi journal of biological sciences · 2020Article
- Article
- Assessment of genetic diversity inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Inter and intraspecific genetic diversity (RAPD) among three most frequent species of macrofungi (Journal, genetic engineering & biotechnology · 2018Article
- Assessment of genetic diversity in Costus pictus accessions based on RAPD and ISSR markers.3 Biotech · 2017Article
- Variations in genetic and chemical constituents ofJournal, genetic engineering & biotechnology · 2016Article
- Development of novel SSR markers for evaluation of genetic diversity and population structure in Tribulus terrestris L. (Zygophyllaceae).3 Biotech · 2016Article
- Genomic profile of the plants with pharmaceutical value.3 Biotech · 2014Review
- Genetic diversity and population structure among pea (Pisum sativum L.) cultivars as revealed by simple sequence repeat and novel genic markers.Molecular biotechnology · 2014Article
- The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers.Physiology and molecular biology of plants : an international journal of functional plant biology · 2012Article
- Estimation of genetic diversity and evaluation of relatedness through molecular markers among medicinally important trees: Terminalia arjuna, T. chebula and T. bellerica.Molecular biology reports · 2011Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tribulus terrestris is well known for its medicinal importance in curing urino-genital disorders. Amplified fragment length polymorphism (AFLP), selective amplification of microsatellite polymorphic loci (SAMPL), inter-simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) markers were used for the first time for the detection of genetic polymorphism in this medicinal herb from samples collected from various geographical regions of India. Six assays each of AFLP and SAMPL markers and 21 each of ISSR and RAPD markers were utilized. AFLP yielded 500 scorable amplified products, of which 82.9% were polymorphic. SAMPL primers amplified 488 bands, 462 being polymorphic (94.7%). The range of amplified bands was 66 [(TC)(8)G + M-CAG] to 98 [(CA)(6)AG + M-CAC] and the percentage polymorphism, 89.9 [from (CT)(4)C (AC)(4)A + M-CTG] to 100 [from (GACA)(4) + M-CTA]. The ISSR primers amplified 239 bands of 0.4-2.5 kb, 73.6% showed polymorphism. The amplified products ranged from 5 to 16 and the percentage polymorphism 40-100. RAPD assays produced 276 bands, of which 163 were polymorphic (59%). Mantel test employed for detection of goodness of fit established cophenetic correlation values above 0.9 for all the four marker systems. The dendrograms and PCA plots derived from the binary data matrices of the four marker systems are highly concordant. High bootstrap values were obtained at major nodes of phenograms through WINBOOT software. The relative efficiency of the four molecular marker systems calculated on the basis of multiplex ratio, marker index and average heterozygosity revealed SAMPL to be the best. Distinct DNA fingerprinting profile, unique to every geographical region could be obtained with all the four molecular marker systems. Clustering can be a good indicator for clear separation of genotypes from different regions in well-defined groups that are supported by high bootstrap values.
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