ArticleNucleic acids research2008
Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 47 citations in OpenAlex.
- Systemic LINE-1 RNA in Plasma Extracellular Vesicles Drives Neuroinflammation and Cognitive Dysfunction via cGAS-STING Pathway in Aging.Aging cell · 2026Article
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- Organ-, sex- and age-dependent patterns of endogenous L1 mRNA expression at a single locus resolution.Nucleic acids research · 2021Article
- Robust expression of LINE-1 retrotransposon encoded proteins in oral squamous cell carcinoma.BMC cancer · 2021Observational
- Cytoplasmic synthesis of endogenousProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Analysis of LINE1 Retrotransposons in Huntington's Disease.Frontiers in cellular neuroscience · 2021Article
- cGAS/STING Pathway Activation Contributes to Delayed Neurodegeneration in Neonatal Hypoxia-Ischemia Rat Model: Possible Involvement of LINE-1.Molecular neurobiology · 2020Article
- Five months of voluntary wheel running downregulates skeletal muscle LINE-1 gene expression in rats.American journal of physiology. Cell physiology · 2019Article
- Identification of charged amino acids required for nuclear localization of human L1 ORF1 protein.Mobile DNA · 2019Article
- Long INterspersed nuclear Elements (LINEs) in brain and non-brain tissues of the rat.Cell and tissue research · 2018Article
- Spontaneous death of rat chloroleukaemia cells induced by an endogenous growth inhibitor.Cell proliferation · 2018Article
- Properties of LINE-1 proteins and repeat element expression in the context of amyotrophic lateral sclerosis.Mobile DNA · 2018Article
- Expression dynamics and relations with nearby genes of rat transposable elements across 11 organs, 4 developmental stages and both sexes.BMC genomics · 2017Article
- Detection of the LINE-1 retrotransposon RNA-binding protein ORF1p in different anatomical regions of the human brain.Mobile DNA · 2017Article
- The Flow of the Gibbon LAVA Element Is Facilitated by the LINE-1 Retrotransposition Machinery.Genome biology and evolution · 2016Article
- LINE-1 distribution in six rodent genomes follow a species-specific pattern.Journal of genetics · 2016Article
- Enhanced expression of LINE-1-encoded ORF2 protein in early stages of colon and prostate transformation.Oncotarget · 2016Article
- Distinct subcellular localization and potential role of LINE1-ORF1P in meiotic oocytes.Histochemistry and cell biology · 2016Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
LINE-1 (L1) is a highly successful autonomous non-LTR retrotransposon and a major force shaping mammalian genomes. Although there are about 600 000 L1 copies covering 23% of the rat genome, full-length rat L1s (L1Rn) with intact open reading frames (ORFs) representing functional master copies for retrotransposition have not been identified yet. In conjunction with studies to elucidate the role of L1 retrotransposons in tumorigenesis, we isolated and characterized 10 different cDNAs from transcribed full-length L1Rn elements in rat chloroleukemia (RCL) cells, each encoding intact ORF1 proteins (ORF1p). We identified the first functional L1Rn retrotransposon from this pool of cDNAs, determined its activity in HeLa cells and in the RCL cell line the cDNAs originated from and demonstrate that it is mobilized in the tumor cell line in which it is expressed. Furthermore, we generated monoclonal antibodies directed against L1Rn ORF1 and ORF2-encoded recombinant proteins, analyzed the expression of L1-encoded proteins and found ORF1p predominantly in the nucleus. Our results support the hypothesis that the reported explosive amplification of genomic L1Rn sequences after their transcriptional activation in RCL cells is based on L1 retrotransposition. Therefore, L1 activity might be one cause for genomic instability observed during the progression of leukemia.
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