ArticleNucleic acids research2008
Protein evolution by hypermutation and selection in the B cell line DT40.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Comparison among bright green fluorescent proteins inmicroPublication biology · 2025Article
- Molecular mechanisms of avian immunoglobulin gene diversification and prospect for industrial applications.Frontiers in immunology · 2024Review
- In vitro evolution predicts emerging SARS-CoV-2 mutations with high affinity for ACE2 and cross-species binding.PLoS pathogens · 2022Article
- Rapid directed molecular evolution of fluorescent proteins in mammalian cells.Protein science : a publication of the Protein Society · 2022Article
- Observing and Quantifying Fluorescent Reporters.Methods in molecular biology (Clifton, N.J.) · 2022Article
- In vitro affinity maturation of broader and more-potent variants of the HIV-1-neutralizing antibody CAP256-VRC26.25.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- GFPnovo2, a brighter GFP variant for in vivo labeling inmicroPublication biology · 2018Article
- Transient AID expression for in situ mutagenesis with improved cellular fitness.Scientific reports · 2018Article
- A Dendritic Guidance Receptor Complex Brings Together Distinct Actin Regulators to Drive Efficient F-Actin Assembly and Branching.Developmental cell · 2018Article
- Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.Molecular cell · 2017Review
- A double-strand break can trigger immunoglobulin gene conversion.Nucleic acids research · 2017Article
- Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells.Nature methods · 2016Article
- Directed evolution of human scFvs in DT40 cells.Protein engineering, design & selection : PEDS · 2016Article
- Article
- RAB-10-Dependent Membrane Transport Is Required for Dendrite Arborization.PLoS genetics · 2015Article
- Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications.Nature protocols · 2014Article
- Targeting of somatic hypermutation by immunoglobulin enhancer and enhancer-like sequences.PLoS biology · 2014Article
- Article
- Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display.The Journal of biological chemistry · 2013Article
- Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM.Nucleic acids research · 2013Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-wide mutations and selection within a population are the basis of natural evolution. A similar process occurs during antibody affinity maturation when immunoglobulin genes are hypermutated and only those B cells which express antibodies of improved antigen-binding specificity are expanded. Protein evolution might be simulated in cell culture, if transgene-specific hypermutation can be combined with the selection of cells carrying beneficial mutations. Here, we describe the optimization of a GFP transgene in the B cell line DT40 by hypermutation and iterative fluorescence activated cell sorting. Artificial evolution in DT40 offers unique advantages and may be easily adapted to other transgenes, if the selection for desirable mutations is feasible.
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