ArticleStructure (London, England : 1993)2007
Quaternary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes.
Article in Structure (London, England : 1993), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Inactive-to-Active Transition of Human Thymidine Kinase 1 Revealed by Molecular Dynamics Simulations.Journal of chemical information and modeling · 2022Article
- Solution structure and functional investigation of human guanylate kinase reveals allosteric networking and a crucial role for the enzyme in cancer.The Journal of biological chemistry · 2019Article
- Cyclic oligomer design with de novo αβ-proteins.Protein science : a publication of the Protein Society · 2017Article
- Article
- Effects of T592 phosphomimetic mutations on tetramer stability and dNTPase activity of SAMHD1 can not explain the retroviral restriction defect.Scientific reports · 2016Article
- Synthesis and evaluation of thymidine kinase 1-targeting carboranyl pyrimidine nucleoside analogs for boron neutron capture therapy of cancer.European journal of medicinal chemistry · 2015Article
- Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major.PLoS neglected tropical diseases · 2015Article
- KAY-2-41, a novel nucleoside analogue inhibitor of orthopoxviruses in vitro and in vivo.Antimicrobial agents and chemotherapy · 2014Article
- N3-substituted thymidine bioconjugates for cancer therapy and imaging.Future medicinal chemistry · 2013Review
- Synthesis of N3-substituted carboranyl thymidine bioconjugates and their evaluation as substrates of recombinant human thymidine kinase 1.European journal of medicinal chemistry · 2013Article
- Quaternary structures of recombinant, cellular, and serum forms of thymidine kinase 1 from dogs and humans.BMC biochemistry · 2012Article
- Synthesis, biological evaluation, and radioiodination of halogenated closo-carboranylthymidine analogues.Inorganic chemistry · 2012Article
- Deprotonated imidodiphosphate in AMPPNP-containing protein structures.Acta crystallographica. Section D, Biological crystallography · 2011Article
- Engineering Kinases to Phosphorylate Nucleoside Analogs for Antiviral and Cancer Therapy.Chimia · 2009Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The human cytosolic thymidine kinase (TK) and structurally related TKs in prokaryotes play a crucial role in the synthesis and regulation of the cellular thymidine triphosphate pool. We report the crystal structures of the TK homotetramer from Thermotoga maritima in four different states: its apo-form, a binary complex with thymidine, as well as the ternary structures with the two substrates (thymidine/AppNHp) and the reaction products (TMP/ADP). In combination with fluorescence spectroscopy and mutagenesis experiments, our results demonstrate that ATP binding is linked to a substantial reorganization of the enzyme quaternary structure, leading to a transition from a closed, inactive conformation to an open, catalytic state. We hypothesize that these structural changes are relevant to enzyme function in situ as part of the catalytic cycle and serve an important role in regulating enzyme activity by amplifying the effects of feedback inhibitor binding.
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