ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2008
Raman nanoparticle probes for antibody-based protein detection in tissues.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 66 citations in OpenAlex.
- Spatially resolved determination of the abundance of the HER2 marker in microscopic breast tumors using targeted SERS imaging.Mikrochimica acta · 2021Article
- SERS-Active Substrate with Collective Amplification Design for Trace Analysis of Pesticides.Nanomaterials (Basel, Switzerland) · 2019Article
- Plasmonic nanoparticle-based expansion microscopy with surface-enhanced Raman and dark-field spectroscopic imaging.Biomedical optics express · 2018Article
- Diverse Applications of Nanomedicine.ACS nano · 2017Review
- Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate.Journal of biological engineering · 2016Article
- Numerical simulation of x-ray luminescence optical tomography for small-animal imaging.Journal of biomedical optics · 2014Article
- Protein analytical assays for diagnosing, monitoring, and choosing treatment for cancer patients.Journal of healthcare engineering · 2012Article
- Raman Labeled Nanoparticles: Characterization of Variability and Improved Method for Unmixing.Journal of Raman spectroscopy : JRS · 2012Article
- Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering.International journal of nanomedicine · 2012Article
- Quantitative ratiometric discrimination between noncancerous and cancerous prostate cells based on neuropilin-1 overexpression.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Bio-imaging, detection and analysis by using nanostructures as SERS substrates.Journal of materials chemistry · 2011Article
- Surface-enhanced Raman scattering (SERS) cytometry.Methods in cell biology · 2011Article
- Imaging with Raman spectroscopy.Current pharmaceutical biotechnology · 2010Review
- Cancer proteomics--an evolving battlefield. Conference on Cancer Proteomics 2009: Mechanistic Insights, Technological Advances & Molecular Medicine.EMBO reports · 2009Article
- Chemical analysis in vivo and in vitro by Raman spectroscopy--from single cells to humans.Current opinion in biotechnology · 2009Review
- Spectral analysis of multiplex Raman probe signatures.ACS nano · 2008Article
- Current trends in nanobiosensor technology.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surface-enhanced Raman scattering (SERS) nanoparticles are emerging as a new approach for optical detection of biomolecules. In a model assay in formalin-fixed paraffin-embedded (FFPE) prostate tissue sections, we detect prostate-specific antigen (PSA) using antibody (Ab) conjugated to composite organic-inorganic nanoparticles (COINs), and we use identical staining protocols to compare COIN-Ab and Alexa-Ab conjugates in adjacent tissue sections. Spectral analysis illustrates the fundamental difference between fluorescence and Raman signatures and accurately extracts COIN probe signals from background autofluorescence. Probe signals are used to generate images of PSA expression on the tissue, and quality measures are presented to characterize the performance of the COIN assay in comparison to Alexa. Staining accuracy (ability to correctly identify PSA expression in epithelial cells) is somewhat less for COIN than Alexa, which is attributed to an elevated false negative rate of the COIN. However, COIN provided signal intensities comparable to Alexa, and good intra-, inter-, and lot-to-lot consistencies. Overall, COIN and Alexa detection reagents possess similar performance with FFPE tissues, supporting the further development of Raman probes for this application. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
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