ArticleInfection and immunity2008
Transcriptome changes in Mycoplasma hyopneumoniae during infection.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Undocked Tails, Mycoplasma-like Lesions and Gastric Ulcers in Slaughtering Pigs: What Connection?Animals : an open access journal from MDPI · 2023Article
- Porcine antibody profiles of 33 Mycoplasma hyopneumoniae fusion proteins from M. hyopneumoniae natural infection but not vaccination.Veterinary medicine and science · 2023Article
- The influence of regulatory elements on Mycoplasma hyopneumoniae 7448 transcriptional response during oxidative stress and heat shock.Molecular biology reports · 2022Article
- Article
- Combined Transcriptome Sequencing ofFrontiers in microbiology · 2020Article
- Quantitative Proteomic Analyses of a Pathogenic Strain and Its Highly Passaged Attenuated Strain ofBioMed research international · 2019Article
- Evaluation of growth and gene expression of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis in defined medium.Molecular biology reports · 2018Article
- Validation of a novel Mho microarray for a comprehensive characterisation of the Mycoplasma hominis action in HeLa cell infection.PloS one · 2017Article
- Article
- Differential expression of Spiroplasma citri surface protein genes in the plant and insect hosts.BMC microbiology · 2016Article
- Measuring Escherichia coli Gene Expression during Human Urinary Tract Infections.Pathogens (Basel, Switzerland) · 2016Article
- Article
- Functional characterization of the principal sigma factor RpoD of phytoplasmas via an in vitro transcription assay.Scientific reports · 2015Article
- Intrinsic terminators in Mycoplasma hyopneumoniae transcription.BMC genomics · 2015Article
- Mycoplasma iowae: relationships among oxygen, virulence, and protection from oxidative stress.Veterinary research · 2015Article
- MG428 is a novel positive regulator of recombination that triggers mgpB and mgpC gene variation in Mycoplasma genitalium.Molecular microbiology · 2014Article
- Genome organization in Mycoplasma hyopneumoniae: identification of promoter-like sequences.Molecular biology reports · 2014Article
- Proteogenomic mapping of Mycoplasma hyopneumoniae virulent strain 232.BMC genomics · 2014Article
- Comparative genomic analyses of Mycoplasma hyopneumoniae pathogenic 168 strain and its high-passaged attenuated strain.BMC genomics · 2013Article
- Transcriptional regulation of MG_149, an osmoinducible lipoprotein gene from Mycoplasma genitalium.Molecular microbiology · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoplasma hyopneumoniae causes swine pneumonia and contributes significantly to the porcine respiratory disease complex. The mechanisms of pathogenesis are difficult to address, since there is a lack of genetic tools, but microarrays are available and can be used to study transcriptional changes that occur during disease as a way to identify important virulence-related genes. Mycoplasmas were collected from bronchial alveolar lavage samples and compared to broth-grown cells using microarrays. Bronchial alveolar lavage was performed on pigs 28 days postinfection, and mycoplasmas were isolated by differential centrifugation. Mycoplasma RNA-enriched preparations were then obtained from total RNA by subtracting eucaryotic ribosomal and messenger RNAs. Labeled cDNAs were generated with mycoplasma open reading frame-specific primers. Nine biological replicates were analyzed. During lung infection, our analysis indicated that 79 M. hyopneumoniae genes were differentially expressed (P < 0.01), at a false-discovery rate of <2.7%. Of the down-regulated genes, 28 of 46 (61%) lacked an assigned function, in comparison to 21 of 33 (63%) of up-regulated genes. Four down-regulated genes and two up-regulated genes encoded putative lipoproteins. secA (mhp295) (P = 0.003) and two glycerol transport permease genes (potA [mhp380; P = 0.006] and ugpA [mhp381; P = 0.003]) were up-regulated in vivo. Elongation factor EF-G (fusA [mhp083]) (P = 0.002), RNA polymerase beta chain (rpoC [mhp635]) (P = 0.003), adenylate kinase (adk [mhp208]) (P = 0.001), prolyl aminoacyl tRNA synthetase (proS [mhp397]) (P = 0.009), and cysteinyl-tRNA synthetase (cysS [mhp661]) (P < 0.001) were down-regulated in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.