ArticleThe Journal of biological chemistry2008
PagP activation in the outer membrane triggers R3 core oligosaccharide truncation in the cytoplasm of Escherichia coli O157:H7.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Antimicrobial Peptides Against Antimicrobial-Resistant Bacteria: Focus on Machine Learning.Infection and drug resistance · 2026Review
- Molecular Mechanisms of Bacterial Resistance to Antimicrobial Peptides in the Modern Era: An Updated Review.Microorganisms · 2024Review
- Co-ordinated assembly of the multilayered cell envelope of Gram-negative bacteria.Current opinion in microbiology · 2024Review
- Regulation ofJournal of bacteriology · 2019Article
- Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing.Nature communications · 2019Article
- Article
- Chemical synthesis of the outer core oligosaccharide of Escherichia coli R3 and immunological evaluation.Organic & biomolecular chemistry · 2015Article
- Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs.BioMed research international · 2015Review
- Deletion of the β-acetoacetyl synthase FabY in Pseudomonas aeruginosa induces hypoacylation of lipopolysaccharide and increases antimicrobial susceptibility.Antimicrobial agents and chemotherapy · 2014Article
- Article
- Inscribing the perimeter of the PagP hydrocarbon ruler by site-specific chemical alkylation.Biochemistry · 2010Article
- PagP crystallized from SDS/cosolvent reveals the route for phospholipid access to the hydrocarbon ruler.Structure (London, England : 1993) · 2010Article
- A thiolate anion buried within the hydrocarbon ruler perturbs PagP lipid acyl chain selection.Biochemistry · 2010Article
- Characterization of BNT2, an intrinsically curved DNA of Escherichia coli O157:H7.Biochemical and biophysical research communications · 2010Article
- Molecular mechanism for lateral lipid diffusion between the outer membrane external leaflet and a beta-barrel hydrocarbon ruler.Biochemistry · 2009Article
- Structural modifications of outer membrane vesicles to refine them as vaccine delivery vehicles.Biochimica et biophysica acta · 2009Article
- Microwave-assisted sample preparation for rapid and sensitive analysis of H. pylori lipid A applicable to a single colony.Journal of lipid research · 2009Article
- All blood, no stool: enterohemorrhagic Escherichia coli O157:H7 infection.Journal of veterinary science · 2008Review
- Extracellular loops of lipid A 3-O-deacylase PagL are involved in recognition of aminoarabinose-based membrane modifications in Salmonella enterica serovar typhimurium.Journal of bacteriology · 2008Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
Abstract
The Escherichia coli outer membrane phospholipid:lipid A palmitoyltransferase PagP is normally a latent enzyme, but it can be directly activated in outer membranes by lipid redistribution associated with a breach in the permeability barrier. We now demonstrate that a lipid A myristate deficiency in an E. coli O157:H7 msbB mutant constitutively activates PagP in outer membranes. The lipid A myristate deficiency is associated with hydrophobic antibiotic sensitivity and, unexpectedly, with serum sensitivity, which resulted from O-antigen polysaccharide absence due to a cytoplasmically determined truncation at the first outer core glucose unit of the R3 core oligosaccharide. Mutational inactivation of pagP in the myristate-deficient lipid A background aggravated the hydrophobic antibiotic sensitivity as a result of losing a partially compensatory increase in lipid A palmitoylation while simultaneously restoring serum resistance and O-antigen attachment to intact lipopolysaccharide. Complementation with either wild-type pagP or catalytically inactive pagPSer77Ala alleles restored the R3 core truncation. However, the intact lipopolysaccharide was preserved after complementation with an internal deletion pagPDelta5-14 allele, which mostly eliminates a periplasmic amphipathic alpha-helical domain but fully supports cell surface lipid A palmitoylation. Our findings indicate that activation of PagP not only triggers lipid A palmitoylation in the outer membrane but also separately truncates the R3 core oligosaccharide in the cytoplasm. We discuss the implication that PagP might function as an apical sensory transducer, which can be activated by a breach in the outer membrane permeability barrier.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.