ArticleJournal of structural biology2008
Expedited approaches to whole cell electron tomography and organelle mark-up in situ in high-pressure frozen pancreatic islets.
Article in Journal of structural biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 142 citations in OpenAlex.
- Three-dimensional ultrastructure of giant mitochondria in human non-alcoholic fatty liver disease.Scientific reports · 2021Trial
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- Structure of a contractile injection system in Salmonella enterica subsp. salamae.Nature communications · 2026Article
- Modular segmentation, spatial analysis and visualization of volume electron microscopy datasets.Nature protocols · 2024Review
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Whole-cell observation of ZIO-stained Golgi apparatus in rat hepatocytes with serial block-face scanning electron microscope, SBF-SEM.Microscopy (Oxford, England) · 2022Article
- Contribution of the Golgi apparatus in morphogenesis of a virus-induced cytopathic vacuolar system.Life science alliance · 2022Article
- Article
- Montage electron tomography of vitrified specimens.Journal of structural biology · 2022Article
- Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.Structure (London, England : 1993) · 2022Article
- CREB3L1 and CREB3L2 control Golgi remodelling during decidualization of endometrial stromal cells.Frontiers in cell and developmental biology · 2022Article
- Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle.The Journal of cell biology · 2021Article
- Article
- Mitochondrial Heterogeneity in Metabolic Diseases.Biology · 2021Review
- Bayesian metamodeling of complex biological systems across varying representations.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- State-of-the-art microscopy to understand islets of Langerhans: what to expect next?Immunology and cell biology · 2021Review
- Article
- 3D FIB-SEM reconstruction of microtubule-organelle interaction in whole primary mouse β cells.The Journal of cell biology · 2021Article
- Visualizing subcellular rearrangements in intact β cells using soft x-ray tomography.Science advances · 2020Article
- Visualizing insulin vesicle neighborhoods in β cells by cryo-electron tomography.Science advances · 2020Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a simplified, efficient approach for the 3D reconstruction and analysis of mammalian cells in toto by electron microscope tomography (ET), to provide quantitative information regarding 'global' cellular organization at approximately 15-20 nm resolution. Two insulin-secreting beta cells-deemed 'functionally equivalent' by virtue of their location at the periphery of the same pancreatic islet-were reconstructed in their entirety in 3D after fast-freezing/freeze-substitution/plastic embedment in situ within a glucose-stimulated islet of Langerhans isolated intact from mouse pancreata. These cellular reconstructions have afforded several unique insights into fundamental structure-function relationships among key organelles involved in the biosynthesis and release of the crucial metabolic hormone, insulin, that could not be provided by other methods. The Golgi ribbon, mitochondria and insulin secretory granules in each cell were segmented for comparative analysis. We propose that relative differences between the two cells in terms of the number, dimensions and spatial distribution (and for mitochondria, also the extent of branching) of these organelles per cubic micron of cellular volume reflects differences in the two cells' individual capacity (and/or readiness) to respond to secretagogue stimulation, reflected by an apparent inverse relationship between the number/size of insulin secretory granules versus the number/size of mitochondria and the Golgi ribbon. We discuss the advantages of this approach for quantitative cellular ET of mammalian cells, briefly discuss its application relevant to other complementary techniques, and summarize future strategies for overcoming some of its current limitations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.