ArticleMolecular microbiology2008
Genomic rearrangements and transcriptional analysis of the spliced leader-associated retrotransposon in RNA interference-deficient Trypanosoma brucei.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Review
- FACT plays a major role in histone dynamics affecting VSG expression site control in Trypanosoma brucei.Molecular microbiology · 2014Article
- Small interfering RNA-producing loci in the ancient parasitic eukaryote Trypanosoma brucei.BMC genomics · 2012Article
- RNA interference in protozoan parasites: achievements and challenges.Eukaryotic cell · 2011Review
- The emerging world of small silencing RNAs in protozoan parasites.Trends in parasitology · 2011Review
- Article
- Small nucleolar RNA interference in Trypanosoma brucei: mechanism and utilization for elucidating the function of snoRNAs.Nucleic acids research · 2010Article
- RNA interference, growth and differentiation appear normal in African trypanosomes lacking Tudor staphylococcal nuclease.Molecular and biochemical parasitology · 2010Article
- Retention and loss of RNA interference pathways in trypanosomatid protozoans.PLoS pathogens · 2010Article
- The FACT subunit TbSpt16 is involved in cell cycle specific control of VSG expression sites in Trypanosoma brucei.Molecular microbiology · 2010Article
- Repetitive elements in parasitic protozoa.BMC biology · 2010Article
- Gene expression in trypanosomatid parasites.Journal of biomedicine & biotechnology · 2010Review
- Active VSG expression sites in Trypanosoma brucei are depleted of nucleosomes.Eukaryotic cell · 2010Article
- Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei.PloS one · 2009Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The Trypanosoma brucei genome is colonized by the site-specific non-LTR retrotransposon SLACS, or spliced leader-associated conserved sequence, which integrates exclusively into the spliced leader (SL) RNA genes. Although there is evidence that the RNA interference (RNAi) machinery regulates SLACS transcript levels, we do not know whether RNAi deficiency affects the genomic stability of SLACS, nor do we understand the mechanism of SLACS transcription. Here, we report that prolonged culturing of RNAi-deficient T. brucei cells, but not wild-type cells, results in genomic rearrangements of SLACS. Furthermore, two populations of SLACS transcripts persist in RNAi-deficient cells: a full-length transcript of approximately 7 kb and a heterogeneous population of small SLACS transcripts ranging in size from 450 to 550 nt. We provide evidence that SLACS transcription initiates at the +1 of the interrupted SL RNA gene and proceeds into the 5' UTR and open reading frame 1 (ORF1). This transcription is carried out by an RNA polymerase with alpha-amanitin sensitivity reminiscent of SL RNA synthesis and is dependent on the SL RNA promoter. Additionally, we show that both sense and antisense small SLACS transcripts originate from ORF1 and that they are associated with proteins in vivo. We speculate that the small SLACS transcripts serve as substrates for the production of siRNAs to regulate SLACS expression.
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