ArticleThe Plant cell2007
Two distinct forms of M-locus protein kinase localize to the plasma membrane and interact directly with S-locus receptor kinase to transduce self-incompatibility signaling in Brassica rapa.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 175 citations in OpenAlex.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Deciphering the Transcriptomic Dynamics of Self-Incompatibility in Yellow Passion Fruit: Evidence of Modified Sporophytic Mechanism.Plants (Basel, Switzerland) · 2026Article
- From recognition to response: integrated signaling pathways determining pollen acceptance and rejection in Brassicaceae.The New phytologist · 2026Review
- Several plant self-incompatibility systems may be controlled by atypical receptor-ligand interactions.The Plant journal : for cell and molecular biology · 2026Review
- Characterization of self-incompatibility genes inFrontiers in plant science · 2026Article
- Site-Directed Mutagenesis Mediated by Molecular Modeling and Docking and Its Effect on the Protein-Protein Interactions of the bHLH Transcription Factors SPATULA, HECATE1, and INDEHISCENT.Plants (Basel, Switzerland) · 2025Article
- A Detailed Protocol for the Isolation of Arabidopsis thaliana Mesophyll Protoplasts and their Applications.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Peptides in plant reproduction-small yet powerful.Frontiers in plant science · 2025Review
- Investigating a role for PUB17 and PUB16 in the self-incompatibility signaling pathway in transgenicPlant direct · 2024Article
- Mapping quantitative trait loci associated with self-(in)compatibility in goji berries (Lycium barbarum).BMC plant biology · 2024Article
- Comparative stigmatic transcriptomics reveals self and cross pollination responses to heteromorphic incompatibility inFrontiers in genetics · 2024Article
- An update on evolutionary, structural, and functional studies of receptor-like kinases in plants.Frontiers in plant science · 2024Review
- Development of self-compatible Chinese cabbage lines of Chiifu through marker-assisted selection.Frontiers in plant science · 2024Article
- Molecular mechanisms of self-incompatibility in Brassicaceae and Solanaceae.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2024Review
- Stop and go signals at the stigma-pollen interface of the Brassicaceae.Plant physiology · 2023Article
- MLPK function is not required for self-incompatibility in the SPlant reproduction · 2023Article
- Self-incompatibility: a targeted, unexplored pre-fertilization barrier in flower crops of Asteraceae.Journal of plant research · 2023Review
- Paradigms of receptor kinase signaling in plants.The Biochemical journal · 2023Review
- Fatty Acid Biosynthesis Pathways Are Downregulated during Stigma Development and Are Critical during Self-Incompatible Responses in Ornamental Kale.International journal of molecular sciences · 2022Article
- A pair of non-Mendelian genes at the Ga2 locus confer unilateral cross-incompatibility in maize.Nature communications · 2022Article
10 more citing papers are in PubMed but not listed here.
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8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many flowering plants possess systems of self-incompatibility (SI) to prevent inbreeding. In Brassica, SI recognition is controlled by the multiallelic gene complex (S-haplotypes) at the S-locus, which encodes both the male determinant S-locus protein 11 (SP11/SCR) and the female determinant S-receptor kinase (SRK). Upon self-pollination, the S-haplotype-specific interaction between the pollen-borne SP11 and the cognate stigmatic SRK receptor induces SI signaling in the stigmatic papilla cell and results in rejection of the self-pollen. Our genetic analysis of a self-compatible mutant revealed the involvement of a cytoplasmic protein kinase, M-locus protein kinase (MLPK), in the SI signaling, but its exact physiological function remains unknown. In this study, we identified two different MLPK transcripts, MLPKf1 and MLPKf2, which are produced using alternative transcriptional initiation sites and encode two isoforms that differ only at the N termini. While MLPKf1 and MLPKf2 exhibited distinct expression profiles, both were expressed in papilla cells. MLPKf1 localizes to the plasma membrane through its N-terminal myristoylation motif, while MLPKf2 localizes to the plasma membrane through its N-terminal hydrophobic region. Although both MLPKf1 and MLPKf2 could independently complement the mlpk/mlpk mutation, their mutant forms that lack the plasma membrane localization motifs failed to complement the mutation. Furthermore, a bimolecular fluorescence complementation assay revealed direct interactions between SRK and the MLPK isoforms in planta. These results suggest that MLPK isoforms localize to the papilla cell membrane and interact directly with SRK to transduce SI signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.