ArticleApplied and environmental microbiology2008
Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?FEMS microbiology reviews · 2023Pooled it
- Advancements in mitigating interference in quantitative polymerase chain reaction (qPCR) for microbial water quality monitoring.The Science of the total environment · 2019Pooled it
- Characterizing fecal bioaerosols in dairy barns using microbial source tracking markers and bovine enteric viruses.Microbiology spectrum · 2026Article
- Optimizing Digital PCR Assays for Multiplexed Microbial Source Tracking in Surface Waters.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bacterial diversity and health hazards associated with resuspended road matter transported by runoff into storm and combined sewers.Water science and technology : a journal of the International Association on Water Pollution Research · 2025Article
- The Impact of the Variability of RT-qPCR Standard Curves on Reliable Viral Detection in Wastewater Surveillance.Microorganisms · 2025Article
- An integrated approach to coupled nutrient and microbial source tracking in an agricultural watershed.Water research · 2025Article
- Beyond borders: A systematic review and meta-analysis of human-specific faecal markers across geographical settings.Critical reviews in environmental science and technology · 2025Review
- Occurrence of recreational water quality monitoring general fecal indicator bacteria and fecal source identification genetic markers in gray seal scat.The Science of the total environment · 2024Article
- Fecal Impairment Framework, A New Conceptual Framework for Assessing Fecal Contamination in Recreational Waters.Environmental management · 2024Article
- Review
- Strategies for Monitoring Microbial Life in Beach Sand for Protection of Public Health.International journal of environmental research and public health · 2023Review
- Exploring the Bacterial Community in Aged Fecal Sources from Dairy Cows: Impacts on Fecal Source Tracking.Microorganisms · 2023Article
- Host-AssociatedMicroorganisms · 2023Article
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Interlaboratory performance and quantitative PCR data acceptance metrics for NIST SRM® 2917.Water research · 2022Article
- Microbial Source Tracking as a Method of Determination of Beach Sand Contamination.International journal of environmental research and public health · 2022Article
- Performance of NIST SRM® 2917 with 13 recreational water quality monitoring qPCR assays.Water research · 2022Article
- Bacteriophages in sewage: abundance, roles, and applications.FEMS microbes · 2022Article
- Tools for interpretation of wastewater SARS-CoV-2 temporal and spatial trends demonstrated with data collected in the San Francisco Bay Area.Water research X · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate assessment of health risks associated with bovine (cattle) fecal pollution requires a reliable host-specific genetic marker and a rapid quantification method. We report the development of quantitative PCR assays for the detection of two recently described bovine feces-specific genetic markers and a method for the enumeration of these markers using a Markov chain Monte Carlo approach. Both assays exhibited a range of quantification from 25 to 2 x 10(6) copies of target DNA, with a coefficient of variation of <2.1%. One of these assays can be multiplexed with an internal amplification control to simultaneously detect the bovine-specific genetic target and presence of amplification inhibitors. The assays detected only cattle fecal specimens when tested against 204 fecal DNA extracts from 16 different animal species and also demonstrated a broad distribution among individual bovine samples (98 to 100%) collected from five geographically distinct locations. The abundance of each bovine-specific genetic marker was measured in 48 individual samples and compared to quantitative PCR-enumerated quantities of rRNA gene sequences representing total Bacteroidetes, Bacteroides thetaiotaomicron, and enterococci in the same specimens. Acceptable assay performance combined with the prevalence of DNA targets across different cattle populations provides experimental evidence that these quantitative assays will be useful in monitoring bovine fecal pollution in ambient waters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.