ArticleJournal of bacteriology2008
Chemotaxis of Escherichia coli to pyrimidines: a new role for the signal transducer tap.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Response ofMicrobiology spectrum · 2025Article
- A Methyl-Accepting Chemotaxis Protein MCP-5685 Associated with Indole Synthesis in Pantoea ananatis YJ76 Influences its Plant Growth-Promoting Potential and Adaptability to Stress Conditions.Current microbiology · 2025Article
- Sensing preferences for prokaryotic solute binding protein families.Microbial biotechnology · 2023Article
- Cellular Effects of 2',3'-Cyclic Nucleotide Monophosphates in Gram-Negative Bacteria.Journal of bacteriology · 2022Article
- RNase I ModulatesACS chemical biology · 2020Article
- Article
- Helicobacter pylori senses bleach (HOCl) as a chemoattractant using a cytosolic chemoreceptor.PLoS biology · 2019Article
- GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli.Microbial cell factories · 2019Article
- Escape band inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Sensory Repertoire of Bacterial Chemoreceptors.Microbiology and molecular biology reviews : MMBR · 2017Review
- The expression of many chemoreceptor genes depends on the cognate chemoeffector as well as on the growth medium and phase.Current genetics · 2017Article
- Extracellular Signals of a Human Epithelial Colorectal Adenocarcinoma (Caco-2) Cell Line Facilitate the Penetration ofFrontiers in cellular and infection microbiology · 2017Article
- Evolutionary Genomics Suggests That CheV Is an Additional Adaptor for Accommodating Specific Chemoreceptors within the Chemotaxis Signaling Complex.PLoS computational biology · 2016Article
- Bacterial chemoreceptors and chemoeffectors.Cellular and molecular life sciences : CMLS · 2015Review
- Article
- Chemoreceptor gene loss and acquisition via horizontal gene transfer in Escherichia coli.Journal of bacteriology · 2013Article
- Phenol sensing by Escherichia coli chemoreceptors: a nonclassical mechanism.Journal of bacteriology · 2011Article
- The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems.Journal of bacteriology · 2010Article
- Bacterial chemotaxis to atrazine and related s-triazines.Applied and environmental microbiology · 2009Article
- Chemotaxis to pyrimidines and identification of a cytosine chemoreceptor in Pseudomonas putida.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli exhibits chemotactic responses to sugars, amino acids, and dipeptides, and the responses are mediated by methyl-accepting chemotaxis proteins (MCPs). Using capillary assays, we demonstrated that Escherichia coli RP437 is attracted to the pyrimidines thymine and uracil and the response was constitutively expressed under all tested growth conditions. All MCP mutants lacking the MCP Tap protein showed no response to pyrimidines, suggesting that Tap, which is known to mediate dipeptide chemotaxis, is required for pyrimidine chemotaxis. In order to confirm the role of Tap in pyrimidine chemotaxis, we constructed chimeric chemoreceptors (Tapsr and Tsrap), in which the periplasmic and cytoplasmic domains of Tap and Tsr were switched. When Tapsr and Tsrap were individually expressed in an E. coli strain lacking all four native MCPs, Tapsr mediated chemotaxis toward pyrimidines and dipeptides, but Tsrap did not complement the chemotaxis defect. The addition of the C-terminal 19 amino acids from Tsr to the C terminus of Tsrap resulted in a functional chemoreceptor that mediated chemotaxis to serine but not pyrimidines or dipeptides. These results indicate that the periplasmic domain of Tap is responsible for detecting pyrimidines and the Tsr signaling domain confers on Tapsr the ability to mediate efficient chemotaxis. A mutant lacking dipeptide binding protein (DBP) was wild type for pyrimidine taxis, indicating that DBP, which is the primary chemoreceptor for dipeptides, is not responsible for detecting pyrimidines. It is not yet known whether Tap detects pyrimidines directly or via an additional chemoreceptor protein.
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