ArticleBiophysical journal2008
Optical measurement of mechanical forces inside short DNA loops.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Cytosine methylation regulates DNA bendability depending on the curvature.Chemical science · 2022Article
- Single-Molecule Methods for Investigating the Double-Stranded DNA Bendability.Molecules and cells · 2022Review
- Determinants of cyclization-decyclization kinetics of short DNA with sticky ends.Nucleic acids research · 2020Article
- Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks.Angewandte Chemie (International ed. in English) · 2015Article
- Atomic force microscopy study of DNA flexibility on short length scales: smooth bending versus kinking.Nucleic acids research · 2014Article
- Probing the elastic limit of DNA bending.Nucleic acids research · 2014Article
- Euler buckling and nonlinear kinking of double-stranded DNA.Nucleic acids research · 2013Article
- Strong bending of the DNA double helix.Nucleic acids research · 2013Review
- The RSC chromatin remodelling ATPase translocates DNA with high force and small step size.The EMBO journal · 2011Article
- Forcing a connection: impacts of single-molecule force spectroscopy on in vivo tension sensing.Biopolymers · 2011Review
- Five challenges to bringing single-molecule force spectroscopy into living cells.Nature methods · 2011Article
- The flexibility of locally melted DNA.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge of the mechanical properties of double-stranded DNA (dsDNA) is essential to understand the role of dsDNA looping in gene regulation and the mechanochemistry of molecular machines that operate on dsDNA. Here, we use a newly developed tool, force sensors with optical readout, to measure the forces inside short, strained loops composed of both dsDNA and single-stranded DNA. By varying the length of the loops and their proportion of dsDNA, it was possible to vary their internal forces from 1 pN to >20 pN. Surprisingly, internal loop forces changed erratically as the amount of dsDNA was increased for a given loop length, with the effect most notable in the smallest loop (57 nucleotides). Monte Carlo simulations based on the helical wormlike chain model accurately predict internal forces when more than half of the loop is dsDNA but fail otherwise. Mismatches engineered into the double-stranded regions increased flexibility, suggesting that Watson-Crick basepaired dsDNA can withstand high compressive forces without recourse to multibase melts. Fluorescence correlation spectroscopy further excluded transient melting (microsecond to millisecond duration) as a mechanism for relief of compressive forces in the tested dsDNAs. DNA loops with integrated force sensors may allow the comprehensive mapping of the elasticity of short dsDNAs as a function of both sequence and salt.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.