ArticleBiophysical journal2008
Probing intranuclear environments at the single-molecule level.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 91 citations in OpenAlex.
- Non-random inter-speckle material transfer mediated by dynamic multiphase interchromatin connections.The EMBO journal · 2026Article
- Amplified MS2 labeling reveals heterogeneous dynamics of RNA granules in the live murine brain.bioRxiv : the preprint server for biology · 2026Article
- Combined SPT and FCS methods reveal a mechanism of RNAP II oversampling in cell nuclei.Scientific reports · 2023Article
- MeCP2-induced heterochromatin organization is driven by oligomerization-based liquid-liquid phase separation and restricted by DNA methylation.Nucleus (Austin, Tex.) · 2022Article
- Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model.Nature communications · 2022Article
- Impact of neurite alignment on organelle motion.Journal of the Royal Society, Interface · 2022Article
- Transcription Factor Dynamics.Cold Spring Harbor perspectives in biology · 2021Review
- Types of nuclear localization signals and mechanisms of protein import into the nucleus.Cell communication and signaling : CCS · 2021Review
- Impact of Nuclear Export Pathway on Cytoplasmic HIV-1 RNA Transport Mechanism and Distribution.mBio · 2020Article
- Visualizing the translation and packaging of HIV-1 full-length RNA.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Processive DNA synthesis is associated with localized decompaction of constitutive heterochromatin at the sites of DNA replication and repair.Nucleus (Austin, Tex.) · 2019Article
- Structure and Dynamics of Mono- vs. Doubly Lipidated Rab5 in Membranes.International journal of molecular sciences · 2019Article
- Recent progress in single-molecule studies of mRNA localizationRNA biology · 2019Review
- A Jump-Distance-Based Parameter Inference Scheme for Particulate Trajectories.Biophysical journal · 2019Article
- Intercalation of small molecules into DNA in chromatin is primarily controlled by superhelical constraint.PloS one · 2019Article
- Formation of Chromatin Subcompartments by Phase Separation.Biophysical journal · 2018Review
- Diatrack particle tracking software: Review of applications and performance evaluation.Traffic (Copenhagen, Denmark) · 2017Review
- Conserved linear dynamics of single-molecule Brownian motion.Nature communications · 2017Article
- 3D Protein Dynamics in the Cell Nucleus.Biophysical journal · 2017Article
- 3D replicon distributions arise from stochastic initiation and domino-like DNA replication progression.Nature communications · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome activity and nuclear metabolism clearly depend on accessibility, but it is not known whether and to what extent nuclear structures limit the mobility and access of individual molecules. We used fluorescently labeled streptavidin with a nuclear localization signal as an average-sized, inert protein to probe the nuclear environment. The protein was injected into the cytoplasm of mouse cells, and single molecules were tracked in the nucleus with high-speed fluorescence microscopy. We analyzed and compared the mobility of single streptavidin molecules in structurally and functionally distinct nuclear compartments of living cells. Our results indicated that all nuclear subcompartments were easily and similarly accessible for such an average-sized protein, and even condensed heterochromatin neither excluded single molecules nor impeded their passage. The only significant difference was a higher frequency of transient trappings in heterochromatin, which lasted only tens of milliseconds. The streptavidin molecules, however, did not accumulate in heterochromatin, suggesting comparatively less free volume. Interestingly, the nucleolus seemed to exclude streptavidin, as it did many other nuclear proteins, when visualized by conventional fluorescence microscopy. The tracking of single molecules, nonetheless, showed no evidence for repulsion at the border but relatively unimpeded passage through the nucleolus. These results clearly show that single-molecule tracking can provide novel insights into mobility of proteins in the nucleus that cannot be obtained by conventional fluorescence microscopy. Our results suggest that nuclear processes may not be regulated at the level of physical accessibility but rather by local concentration of reactants and availability of binding sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.