ArticleJournal of mass spectrometry : JMS2008
Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry.
Article in Journal of mass spectrometry : JMS, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Relative Quantitation of EFNA1 Expression in Mouse Heart Tissue Histologic Sections Using MALDI-MSI.International journal of molecular sciences · 2025Article
- Automated sample preparation in a microfluidic culture device for cellular metabolomics.The Analyst · 2016Article
- FLEXITau: Quantifying Post-translational Modifications of Tau Protein in Vitro and in Human Disease.Analytical chemistry · 2016Article
- A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity.Chemical communications (Cambridge, England) · 2015Article
- Studies on quantitative phosphopeptide analysis by matrix-assisted laser desorption/ionization mass spectrometry without label, chromatography or calibration curves.Rapid communications in mass spectrometry : RCM · 2014Article
- Probing enzymatic activity inside living cells using a nanowire-cell "sandwich" assay.Nano letters · 2013Article
- Photocleavable peptide-oligonucleotide conjugates for protein kinase assays by MALDI-TOF MS.Molecular bioSystems · 2012Article
- A magnetic bead-based protein kinase assay with dual detection techniques.Analytical biochemistry · 2011Article
- Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS.Bioconjugate chemistry · 2010Article
- A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Analytical biochemistry · 2010Article
- Sites of intra- and intermolecular cross-linking of the N-terminal extension of troponin I in human cardiac whole troponin complex.The Journal of biological chemistry · 2009Article
- Quantitative matrix-assisted laser desorption/ionization mass spectrometry.Briefings in functional genomics & proteomics · 2008Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Despite advances in methods and instrumentation for analysis of phosphopeptides using mass spectrometry, it is still difficult to quantify the extent of phosphorylation of a substrate because of physiochemical differences between unphosphorylated and phosphorylated peptides. Here we report experiments to investigate those differences using MALDI-TOF mass spectrometry for a set of synthetic peptides by creating calibration curves of known input ratios of peptides/phosphopeptides and analyzing their resulting signal intensity ratios. These calibration curves reveal subtleties in sequence-dependent differences for relative desorption/ionization efficiencies that cannot be seen from single-point calibrations. We found that the behaviors were reproducible with a variability of 5-10% for observed phosphopeptide signal. Although these data allow us to begin addressing the issues related to modeling these properties and predicting relative signal strengths for other peptide sequences, it is clear that this behavior is highly complex and needs to be further explored.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.