ArticleTransgenic research2008
A high-throughput Agrobacterium-mediated transformation system for the grass model species Brachypodium distachyon L.
Article in Transgenic research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- ImprovingGenes · 2023Article
- Transcriptional repression of TaNOX10 by TaWRKY19 compromises ROS generation and enhances wheat susceptibility to stripe rust.The Plant cell · 2022Article
- Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter.Plant methods · 2021Article
- An efficient Agrobacterium-mediated genetic transformation method for foxtail millet (Setaria italica L.).Plant cell reports · 2020Article
- In Vitro Tissue Culture inInternational journal of molecular sciences · 2020Review
- A CRISPR/Cas9-Based Mutagenesis Protocol forFrontiers in plant science · 2020Article
- An Efficient System forPlant physiology · 2019Article
- Review
- Brachypodium distachyon - A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH.PloS one · 2017Article
- Specific peroxidases differentiate Brachypodium distachyon accessions and are associated with drought tolerance traits.Annals of botany · 2016Article
- A simple and highly efficientBiotechnology reports (Amsterdam, Netherlands) · 2015Article
- Differential gene expression and metabolomic analyses of Brachypodium distachyon infected by deoxynivalenol producing and non-producing strains of Fusarium graminearum.BMC genomics · 2014Article
- Article
- The WRKY transcription factor family in Brachypodium distachyon.BMC genomics · 2012Article
- A comprehensive overview of grain development in Brachypodium distachyon variety Bd21.Journal of experimental botany · 2012Article
- Cell wall composition throughout development for the model grass Brachypodium distachyon.Frontiers in plant science · 2012Article
- Brachypodium as a model for the grasses: today and the future.Plant physiology · 2011Article
- Construction of high quality Gateway™ entry libraries and their application to yeast two-hybrid for the monocot model plant Brachypodium distachyon.BMC biotechnology · 2011Article
- A BAC-based physical map of Brachypodium distachyon and its comparative analysis with rice and wheat.BMC genomics · 2009Article
- Sexually mature transgenic American chestnut trees via embryogenic suspension-based transformation.Plant cell reports · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the ongoing process of developing Brachypodium distachyon as a model plant for temperate cereals and forage grasses, we have developed a high-throughput Agrobacterium-mediated transformation system for a diploid accession. Embryogenic callus, derived from immature embryos of the accession BDR018, were transformed with Agrobacterium tumefaciens strain AGL1 carrying two T-DNA plasmids, pDM805 and pWBV-Ds-Ubi-bar-Ds. Transient and stable transformation efficiencies were optimised by varying the pre-cultivation period, which had a strong effect on stable transformation efficiency. On average 55% of 17-day-old calli co-inoculated with Agrobacterium regenerated stable transgenic plants. Stable transformation frequencies of up to 80%, which to our knowledge is the highest transformation efficiency reported in graminaceous species, were observed. In a study of 177 transgenic lines transformed with pDM805, all of the regenerated transgenic lines were resistant to BASTA, while the gusA gene was expressed in 88% of the transgenic lines. Southern blot analysis revealed that 35% of the tested plants had a single T-DNA integration. Segregation analysis performed on progenies of ten selected T(0) plants indicated simple Mendelian inheritance of the two transgenes. Furthermore, the presence of two selection marker genes, bar and hpt, on the T-DNA of pWBV-Ds-Ubi-bar-Ds allowed us to characterize the developed transformation protocol with respect to full-length integration rate. Even when not selected for, full-length integration occurred in 97% of the transformants when using bialaphos as selection agent.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.