ArticleAnalytical chemistry2008
Initial synthesis and characterization of an alpha7 nicotinic receptor cellular membrane affinity chromatography column: effect of receptor subtype and cell type.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 30 citations in OpenAlex.
- Drug discovery from natural products - Old problems and novel solutions for the treatment of neurodegenerative diseases.Journal of pharmaceutical and biomedical analysis · 2022Review
- Comparison of analytical techniques for the identification of bioactive compounds from natural products.Natural product reports · 2016Review
- Multiple protein stationary phases: a review.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Review
- Comparative normal/failing rat myocardium cell membrane chromatographic analysis system for screening specific components that counteract doxorubicin-induced heart failure from Acontium carmichaeli.Analytical chemistry · 2014Article
- Identification of metabolites of propyrisulfuron in rats.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2014Article
- In vitro and in vivo characterization of a novel negative allosteric modulator of neuronal nAChRs.Neuropharmacology · 2010Article
- MD-354 selectively antagonizes the antinociceptive effects of (-)nicotine in the mouse tail-flick assay.Psychopharmacology · 2010Article
- The preparation and development of cellular membrane affinity chromatography columns.Nature protocols · 2009Article
- Polyethylene glycol-based homologated ligands for nicotinic acetylcholine receptors.Bioorganic & medicinal chemistry · 2008Article
- 3'-Fluoro substitution in the pyridine ring of epibatidine improves selectivity and efficacy for alpha4beta2 versus alpha3beta4 nAChRs.Neuropharmacology · 2008Article
- Characterization of a multiple ligand-gated ion channel cellular membrane affinity chromatography column and identification of endogenously expressed receptors in astrocytoma cell lines.Analytical chemistry · 2008Article
- Investigation of molecular recognition in biological systems using cellular membrane affinity chromatography.Chimica oggi · 2008Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
In this study, cellular membrane fragments from SH-EP1-pCEP4-halpha7 and alpha7 HEK-293 cell lines were used to synthesize cellular membrane affinity chromatography (CMAC) columns containing functional alpha7 nicotinic acetylcholine receptors, CMAC(alpha7 nAChR) columns. The synthesis of stable columns required the addition of cholesterol to the 2% cholate solubilization/immobilization (s/i) buffer and to the mobile phase. In addition, when membranes from the SH-EP1 cell line were used, l-alpha-phosphatidylserine and l-alpha-phosphatidylethanolamine also had to be added to the s/i buffer. A CMAC(alpha4beta2 nAChR) column was prepared using membrane fragments from a SH-EP1-pCEP4-halpha4beta2 cell line, and this process required the addition of l-alpha-phosphatidylserine and l-alpha-phosphatidylethanolamine to the s/i buffer, but not cholesterol. The s/i buffers from the three columns were compared with the s/i buffer utilized in the preparation of a CMAC(alpha4beta2 nAChR) column prepared using an alpha4beta2 HEK-293 cell line, which required no additions to the 2% cholate s/i buffer. The data demonstrate that both cell type and receptor type affect the protocol required to produce a stable CMAC column and that, at the current time, the development of an optimum immobilization protocol is an empirical process. The results are also consistent with the observation that the alpha7 nAChR is localized in lipid rafts in both of these cell lines and that the cholate detergent removed cholesterol from these microdomains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.