ArticleFolia microbiologica2007
Phenotypic and genotypic analysis of Borrelia spp. isolated from Ixodes ricinus ticks by using electrophoretic chips and real-time polymerase chain reaction.
Article in Folia microbiologica, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- Assessment of occupational exposure of soldiers to Lyme disease and Borrelia miyamotoi disease in selected military training areas from northern Poland.Experimental & applied acarology · 2024Article
- Red Foxes (Pathogens (Basel, Switzerland) · 2022Article
- Ticks and the city - are there any differences between city parks and natural forests in terms of tick abundance and prevalence of spirochaetes?Parasites & vectors · 2017Article
- Borrelia miyamotoi and Co-Infection with Borrelia afzelii in Ixodes ricinus Ticks and Rodents from Slovakia.Microbial ecology · 2017Article
- Host Immune Evasion by Lyme and Relapsing Fever Borreliae: Findings to Lead Future Studies forFrontiers in immunology · 2017Review
- Article
- Prevalence of Borrelia miyamotoi in Ixodes ticks in Europe and the United States.Emerging infectious diseases · 2014Article
- Seasonality of Ixodes ricinus ticks on vegetation and on rodents and Borrelia burgdorferi sensu lato genospecies diversity in two Lyme borreliosis-endemic areas in Switzerland.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2012Article
- Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2011Review
- Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii.Ticks and tick-borne diseases · 2010Article
- Establishment of a minor groove binder-probe based quantitative real time PCR to detect Borrelia burgdorferi sensu lato and differentiation of Borrelia spielmanii by ospA-specific conventional PCR.Parasites & vectors · 2010Article
- Genotypic variation and mixtures of Lyme Borrelia in Ixodes ticks from North America and Europe.PloS one · 2010Article
- Delineation of a new species of the Borrelia burgdorferi Sensu Lato Complex, Borrelia americana sp. nov.Journal of clinical microbiology · 2009Article
- Niche partitioning of Borrelia burgdorferi and Borrelia miyamotoi in the same tick vector and mammalian reservoir species.The American journal of tropical medicine and hygiene · 2009Article
- 16S rRNA gene-based identification of cultured bacterial flora from host-seeking Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna ticks, vectors of vertebrate pathogens.Folia microbiologica · 2009Article
- Identification of Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in patients with erythema migrans.Folia microbiologica · 2009Article
- Serological and molecular detection of Borrelia burgdorferi sensu lato and Anaplasmataceae in rodents.Folia microbiologica · 2008Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genotype of Borrelia burgdorferi sensu lato was detected in 371 out of 1244 ticks. Borrelia determination was based on partial sequencing of the 16S rRNA gene and real-time polymerase chain reactions for identification and quantitation of ospA and recA genes. Different Borrelia spp. were identified; B. garinii in 40% ticks followed by B. afzelii (36.3%), B. burgdorferi sensu stricto (12.9%), B. valaisiana (3.5%), B. lusitaniae (0.8%), B. bissettii (0.5%) and B. miyamotoi-like (0.5%). Cultivation of 30 borrelia strains in BSK-H medium, among them B. valaisiana, B. bissettii-like and B. miyamotoi-like strains was unique in Czechia. Calibrated microfluidic-based quantification showed differences in the concentration of the nucleic acids and molar mass of the outer surface proteins of different Borrelia spp. with standard sensitivity and specificity and was helpful for their identification. The outer surface protein OspA was absent in B. miyamotoi-like and the OspB protein in B. valaisiana, B. lusitaniae and in three subtypes of B. garinii.
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