ArticleBiochemical and biophysical research communications2008
The B2 alternatively spliced isoform of nonmuscle myosin II-B lacks actin-activated MgATPase activity and in vitro motility.
Article in Biochemical and biophysical research communications, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 23 citations in OpenAlex.
- A song of heads and tails: myosin II conformational regulation and filament dynamics shape force generation in non-muscle cells.Biophysical reviews · 2026Review
- Structure, regulation, and mechanisms of nonmuscle myosin-2.Cellular and molecular life sciences : CMLS · 2024Review
- Non-muscle myosin 2 at a glance.Journal of cell science · 2023Review
- Review
- Plastic Deformation and Fragmentation of Strained Actin Filaments.Biophysical journal · 2019Article
- Non-muscle Myosin II: Role in Microbial Infection and Its Potential as a Therapeutic Target.Frontiers in microbiology · 2019Review
- Mechanical heterogeneity favors fragmentation of strained actin filaments.Biophysical journal · 2015Article
- Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments.Current biology : CB · 2015Article
- The role of vertebrate nonmuscle Myosin II in development and human disease.Bioarchitecture · 2014Review
- Characterization of three full-length human nonmuscle myosin II paralogs.The Journal of biological chemistry · 2013Article
- Kinetic characterization of the ATPase and actin-activated ATPase activities of Acanthamoeba castellanii myosin-2.The Journal of biological chemistry · 2013Article
- Myosin motors at neuronal synapses: drivers of membrane transport and actin dynamics.Nature reviews. Neuroscience · 2013Review
- Nonmuscle myosin-2: mix and match.Cellular and molecular life sciences : CMLS · 2013Review
- Nonmuscle myosin II is required for internalization of the epidermal growth factor receptor and modulation of downstream signaling.The Journal of biological chemistry · 2012Article
- In vivo studies on nonmuscle myosin II expression and function in heart development.Frontiers in bioscience (Landmark edition) · 2012Review
- Ablation of nonmuscle myosin II-B and II-C reveals a role for nonmuscle myosin II in cardiac myocyte karyokinesis.Molecular biology of the cell · 2010Article
- An alternatively spliced isoform of non-muscle myosin II-C is not regulated by myosin light chain phosphorylation.The Journal of biological chemistry · 2009Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We report the initial biochemical characterization of an alternatively spliced isoform of nonmuscle heavy meromyosin (HMM) II-B2 and compare it with HMM II-B0, the nonspliced isoform. HMM II-B2 is the HMM derivative of an alternatively spliced isoform of endogenous nonmuscle myosin (NM) II-B, which has 21-amino acids inserted into loop 2, near the actin-binding region. NM II-B2 is expressed in the Purkinje cells of the cerebellum as well as in other neuronal cells [X. Ma, S. Kawamoto, J. Uribe, R.S. Adelstein, Function of the neuron-specific alternatively spliced isoforms of nonmuscle myosin II-B during mouse brain development, Mol. Biol. Cell 15 (2006) 2138-2149]. In contrast to any of the previously described isoforms of NM II (II-A, II-B0, II-B1, II-C0 and II-C1) or to smooth muscle myosin, the actin-activated MgATPase activity of HMM II-B2 is not significantly increased from a low, basal level by phosphorylation of the 20kDa myosin light chain (MLC-20). Moreover, although HMM II-B2 can bind to actin in the absence of ATP and is released in its presence, it cannot propel actin in the sliding actin filament assay following MLC-20 phosphorylation. Unlike HMM II-B2, the actin-activated MgATPase activity of a chimeric HMM with the 21-amino acid II-B2 sequence inserted into the homologous location in the heavy chain of HMM II-C is increased following MLC-20 phosphorylation. This indicates that the effect of the II-B2 insert is myosin heavy chain specific.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.