ArticleParasitology research2008
CP2 gene as a useful viability marker for Cryptosporidium parvum.
Article in Parasitology research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Persistence of protozoan parasites andApplied and environmental microbiology · 2026Article
- Use of Oxidative Stress Responses to Determine the Efficacy of Inactivation Treatments onMicroorganisms · 2021Article
- Assessing viability and infectivity of foodborne and waterborne stages (cysts/oocysts) of Giardia duodenalis, Cryptosporidium spp., and Toxoplasma gondii: a review of methods.Parasite (Paris, France) · 2018Review
- Simultaneous Molecular Detection ofThe Korean journal of parasitology · 2017Article
- Detection of Encephalitozoon spp. from human diarrheal stool and farm soil samples in Korea.Journal of Korean medical science · 2015Article
- A nanotherapy strategy significantly enhances anticryptosporidial activity of an inhibitor of bifunctional thymidylate synthase-dihydrofolate reductase from Cryptosporidium.Bioorganic & medicinal chemistry letters · 2015Article
- Detection of Cryptosporidium parvum in environmental soil and vegetables.Journal of Korean medical science · 2014Article
- Cryptosporidium hominis infection diagnosed by real-time PCR-RFLP.The Korean journal of parasitology · 2013Article
- Detection of viable Cryptosporidium parvum in soil by reverse transcription-real-time PCR targeting hsp70 mRNA.Applied and environmental microbiology · 2011Article
- Multiplex PCR detection of waterborne intestinal protozoa: microsporidia, Cyclospora, and Cryptosporidium.The Korean journal of parasitology · 2010Article
- Comparative sensitivity of PCR primer sets for detection of Cryptosporidium parvum.The Korean journal of parasitology · 2009Article
- Overview of Cryptosporidium presentations at the 10th International Workshops on Opportunistic Protists.Eukaryotic cell · 2009Article
- Quantitative evaluation of infectivity change of Cryptosporidium parvum after gamma irradiation.The Korean journal of parasitology · 2009Article
- Pulsed-UV light inactivation of Cryptosporidium parvum.Parasitology research · 2008Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The validity of the CP2 gene of Cryptosporidium parvum as a viability marker was evaluated using absolute quantitative real-time polymerase chain reaction (qPCR) assays. Total ribonucleic acid (RNA) was isolated from live and heat-killed C. parvum oocysts, and complementary deoxyribonucleic acid was synthesized and used as a template. The most accurate number of viable C. parvum oocysts was predicted when the CP2 gene was used as a target gene. The lower detection limit of the CP2 gene was ten oocysts, which was the most sensitive among examined target genes. With heat shock induction, only hsp70 messenger RNA (mRNA) was induced, and the predicted viable oocyst number was increased by heat shock for this marker. The CP2, hsp70, Cryptosporidium oocyst wall protein, and beta-tubulin mRNAs were not detected in heat-killed oocysts, but the 18S ribosomal ribonucleic acid (rRNA) showed heat stability until 48 h after heat killing. Although the 18S rRNA demonstrated the fastest response in crossing point (CP) value among the examined primer sets in qPCR, overestimation of viable oocysts was noted in the analysis with this gene. In conclusion, the CP2 gene was identified as the most sensitive, reliable, and accurate candidate of a viability marker of C. parvum by qPCR evaluation.
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