ArticleCurrent genetics2008
Hallmarks of RNA silencing are found in the smut fungus Ustilago hordei but not in its close relative Ustilago maydis.
Article in Current genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 83 citations in OpenAlex.
- Small RNAs as systemic signals in plant defense: mechanisms, challenges, and future directions.Molecular biology reports · 2026Review
- Cross-kingdom RNAi: a universal mechanism of inter-organismal communication with many unknowns.Journal of experimental botany · 2026Review
- Learning from fungicide resistance: Evolutionary insights to guide RNAi-based control of fungal crop pathogens.Fungal biology reviews · 2025Article
- The Silent Conversation: How Small RNAs Shape Plant-Microbe Relationships.International journal of molecular sciences · 2025Review
- Distinct evolutionary trajectories following loss of RNA interference inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Distinct evolutionary trajectories following loss of RNA interference inbioRxiv : the preprint server for biology · 2024Article
- RNAi-Based Approaches to Control Mycotoxin Producers: Challenges and Perspectives.Journal of fungi (Basel, Switzerland) · 2024Review
- Exploring the challenges of RNAi-based strategies for crop protection.Advanced biotechnology · 2024Review
- What are the 100 most cited fungal genera?Studies in mycology · 2024Article
- Extracellular RNAs released by plant-associated fungi: from fundamental mechanisms to biotechnological applications.Applied microbiology and biotechnology · 2023Review
- A perspective on cross-kingdom RNA interference in mutualistic symbioses.The New phytologist · 2023Review
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities.Functional & integrative genomics · 2023Review
- Control of white mold (Sclerotinia sclerotiorum) through plant-mediated RNA interference.Scientific reports · 2023Article
- Heterochromatin and RNAi act independently to ensure genome stability in Mucorales human fungal pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene.RNA biology · 2023Article
- Roles of RNA silencing in viral and non-viral plant immunity and in the crosstalk between disease resistance systems.Nature reviews. Molecular cell biology · 2022Review
- Management of Post-Harvest Anthracnose: Current Approaches and Future Perspectives.Plants (Basel, Switzerland) · 2022Review
- RNAi as a Foliar Spray: Efficiency and Challenges to Field Applications.International journal of molecular sciences · 2022Review
- Genetic Characteristics and Metabolic Interactions betweenPlants (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) acts through transcriptional and post-transcriptional gene silencing of homologous sequences. With the goal of using RNAi as a tool for studying gene function in the related basidiomycete cereal pathogens Ustilago hordei and Ustilago maydis, we developed a general purpose RNAi expression vector. Tandem, inverted fragments of the GUS gene were inserted into this vector flanking an intron and used to transform engineered GUS-expressing haploid cells. Down-regulation of the GUS gene and production of siRNAs were seen only in U. hordei, even though corresponding GUS double-stranded RNA was detected in both species. Similarly, when the endogenous bW mating-type gene was targeted by RNAi, mating was reduced only in U. hordei. Our work demonstrates the feasibility of using RNAi in U. hordei and provides experimental support for the observed lack of RNAi components in the U. maydis genome. We hypothesize that the sharply limited transposon complement in U. maydis is a biological consequence of this absence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.