ArticlePLoS neglected tropical diseases2007
Cost-effectiveness of blood agar for isolation of mycobacteria.
Article in PLoS neglected tropical diseases, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 61 citations in OpenAlex.
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- A Rare Case of Osteomyelitis of an Ankle Caused byAntibiotics (Basel, Switzerland) · 2023Article
- Bloodstream infections with rapidly growing nontuberculous mycobacteria.Journal of clinical tuberculosis and other mycobacterial diseases · 2021Article
- Comparison of Strategies for Isolating Anaerobic Bacteria from the Porcine Intestine.Applied and environmental microbiology · 2021Article
- An improved simple method for the identification of Mycobacteria by MALDI-TOF MS (Matrix-Assisted Laser Desorption- Ionization mass spectrometry).Scientific reports · 2019Article
- Molecular strategy for the diagnosis of infectious lymphadenitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Overexpression of Adenylyl Cyclase Encoded by theFrontiers in cellular and infection microbiology · 2017Article
- Evaluation of Culture Media for Isolation of Mycobacterium Species from Human Clinical Specimens.Cureus · 2016Article
- Prosthetic joint infection caused by Pasteurella multocida: a case series and review of literature.BMC infectious diseases · 2016Review
- Mycobacterium and Aerobic Actinomycete Culture: Are Two Medium Types and Extended Incubation Times Necessary?Journal of clinical microbiology · 2016Article
- Laryngeal tuberculosis diagnosed by stool sample cultures: a case report.Journal of medical case reports · 2015Article
- Rapid culture-based diagnosis of pulmonary tuberculosis in developed and developing countries.Frontiers in microbiology · 2015Review
- Article
- Current and past strategies for bacterial culture in clinical microbiology.Clinical microbiology reviews · 2015Review
- Bacterial lymphadenitis at a major referral hospital in France from 2008 to 2012.Journal of clinical microbiology · 2014Article
- Dramatic reduction of culture time of Mycobacterium tuberculosis.Scientific reports · 2014Article
- Evaluation of agar-based medium with sheep sera for testing of drug susceptibility of Mycobacterium tuberculosis to Isoniazid, Rifampin, Ethambutol, and Streptomycin.Journal of clinical microbiology · 2013Article
- Cell extract-containing medium for culture of intracellular fastidious bacteria.Journal of clinical microbiology · 2013Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycobacterium species are grown using specific media that increase laboratory cost, thus hampering their diffusion in resource-limited countries. Preliminary data suggested that versatile blood agar may be also used for mycobacterial culture. METHODOLOGY: We examined the growth of 41 different Mycobacterium species on 5% blood agar. Over a 24-month period we analysed isolation of mycobacteria after parallel inoculation of clinical specimens into both a reference automated system (BACTEC 9000 MB broth) and 5% blood agar slant tubes, after NaOH decontamination, and compared the cost of performing 1,000 analyses using these two techniques.
conclusionsMycobacterium reference species cultured on blood agar, with the exception of Mycobacterium ulcerans. Inoculation of 1,634 specimens yielded 95 Mycobacterium isolates. Blood agar performed significantly more efficiently than BACTEC 9000 MB broth (94 vs 88 isolates, P = 0.03). Decontamination of Candida albicans in 5 specimens by addition of amphotericin B in blood agar yielded one more M. tuberculosis isolate that could not be isolated in BACTEC broth. Uneven distribution of time to culture positivity for M. tuberculosis had a median (range) of 19+/-5 days using blood agar and 26+/-6 days using BACTEC 9000 MB broth. Cost for 1,000 analyses in France was estimated to be of 1,913 euros using the blood agar method and 8,990 euros using the BACTEC 9000 MB method. Blood agar should be regarded as a first-line medium for culturing Mycobacterium species. It saves time, is cost-effective, is more sensitive than, and at least as rapid as the automated method. This is of particular importance for resource-limited countries in which the prevalence of tuberculosis is high.
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