ArticlePLoS neglected tropical diseases2007
Detection of Schistosoma mansoni eggs in feces through their interaction with paramagnetic beads in a magnetic field.
Article in PLoS neglected tropical diseases, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 78 citations in OpenAlex.
- The Evaluation of Surface Charges of Ascaris Lumbricoides, Giardia Duodenalis, and Taenia spp., in Preserved Fecal Samples Processed in Natura.Acta parasitologica · 2026Article
- Miracidia as main source for autofluorescence of Schistosoma mansoni eggsMemorias do Instituto Oswaldo Cruz · 2026Article
- Surface Charge-Dependent Targeting and Penetration of Magnetic Nanoparticles into Eggs and Adult Worms ofPharmaceutics · 2026Article
- Magnetic and electromagnetic methods in reducing microbial contamination in water: A systematic review and meta-analyses.One health (Amsterdam, Netherlands) · 2025Review
- Laboratory validation of the automated diagnosis of intestinal parasites via fecal sample processing for the recovery of intestinal parasites through the dissolved air flotation technique.Parasites & vectors · 2024Article
- Development and evaluation of an indirect ELISA using a multiepitope antigen for the diagnosis of intestinal schistosomiasis.Parasitology · 2023Article
- Accuracy Study of Kato-Katz and Helmintex Methods for Diagnosis of Schistosomiasis Mansoni in a Moderate Endemicity Area in Sergipe, Northeastern Brazil.Diagnostics (Basel, Switzerland) · 2023Article
- The Road to Elimination: Current State of Schistosomiasis Research and Progress Towards the End Game.Frontiers in immunology · 2022Review
- Previous History of American Tegumentary Leishmaniasis Alters Susceptibility and Immune Response AgainstFrontiers in immunology · 2021Article
- A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method.PLoS neglected tropical diseases · 2020Article
- A historical review of the techniques of recovery of parasites for their detection in human stools.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- Schistosomiasis: an epidemiological update on Brazil's southernmost low endemic area in Esteio.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- The use of the circulating cathodic antigen (CCA) urine cassette assay for the diagnosis and assessment of cure of Schistosoma mansoni infections in an endemic area of the Amazon region.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- Diagnosis of Schistosoma mansoni infections: what are the choices in Brazilian low-endemic areas?Memorias do Instituto Oswaldo Cruz · 2019Review
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Performance of an Ultra-Sensitive Assay Targeting the Circulating Anodic Antigen (CAA) for Detection ofFrontiers in immunology · 2019Article
- Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin.Memorias do Instituto Oswaldo Cruz · 2019Article
- Study of diagnostic accuracy of Helmintex, Kato-Katz, and POC-CCA methods for diagnosing intestinal schistosomiasis in Candeal, a low intensity transmission area in northeastern Brazil.PLoS neglected tropical diseases · 2018Article
- Evaluation of diagnostic methods for the detection of intestinal schistosomiasis in endemic areas with low parasite loads: Saline gradient, Helmintex, Kato-Katz and rapid urine test.PLoS neglected tropical diseases · 2018Article
- Modulation of Allergic Reactivity in Humans Is Dependent onFrontiers in immunology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDiagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of an established standard with maximum sensitivity and specificity. One strategy to optimize method performance, where eggs are rare events, is to examine large amounts of feces. A novel diagnostic method for isolation of Schistosoma mansoni eggs in feces, and an initial evaluation of its performance is reported here. METHODOLOGY/PRINCIPAL
findingsKnown amounts of S. mansoni eggs were seeded into 30 g of normal human feces and subjected to a sequence of spontaneous sedimentation, sieving, Ritchie method, incubation and isolation through interaction with paramagnetic beads. Preliminary tests demonstrated the efficacy of lectins as ligands, but they also indicated that the paramagnetic beads alone were sufficient to isolate the eggs under a magnetic field through an unknown mechanism. Eggs were identified by microscopic inspection, with a sensitivity of 100% at 1.3 eggs per gram of feces (epg). Sensitivity gradually decreased to 25% at a concentration of 0.1 epg. In a preliminary application of the new method to the investigation of a recently established focus in southern Brazil, approximately 3 times more eggs were detected than with the thick-smear Kato-Katz method. CONCLUSIONS/SIGNIFICANCE: The novel S. mansoni detection method may significantly improve diagnosis of infections with low burdens in areas of recent introduction of the parasite, areas under successful control of transmission, or in infected travelers. It may also improve the evaluation of new treatments and vaccines.
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