ArticleThe EMBO journal2008
Parallel topology of genetically fused EmrE homodimers.
Article in The EMBO journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- Transmembrane helices mediate the formation of a stable ternary complex of bCommunications biology · 2022Article
- Single-Molecule FRET of Membrane Transport Proteins.Chembiochem : a European journal of chemical biology · 2021Review
- Biocide Selective TolC-Independent Efflux Pumps in Enterobacteriaceae.The Journal of membrane biology · 2018Article
- Stable membrane orientations of small dual-topology membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Competition as a way of life for H(+)-coupled antiporters.Journal of molecular biology · 2014Review
- Article
- Essential amino acid residues of BioY reveal that dimers are the functional S unit of the Rhodobacter capsulatus biotin transporter.Journal of bacteriology · 2012Article
- Antiparallel dimers of the small multidrug resistance protein EmrE are more stable than parallel dimers.The Journal of biological chemistry · 2012Article
- Undecided membrane proteins insert in random topologies. Up, down and sideways: it does not really matter.Trends in biochemical sciences · 2012Article
- Analyzing conformational changes in the transport cycle of EmrE.Current opinion in structural biology · 2012Review
- Article
- The role of trimerization in the osmoregulated betaine transporter BetP.EMBO reports · 2011Article
- Structural perspectives on secondary active transporters.Trends in pharmacological sciences · 2010Review
- Structure, dynamics, and substrate-induced conformational changes of the multidrug transporter EmrE in liposomes.The Journal of biological chemistry · 2010Article
- Topologically random insertion of EmrE supports a pathway for evolution of inverted repeats in ion-coupled transporters.The Journal of biological chemistry · 2010Article
- Review
- Diversity and evolution of the small multidrug resistance protein family.BMC evolutionary biology · 2009Article
- The assembly motif of a bacterial small multidrug resistance protein.The Journal of biological chemistry · 2009Article
- An emerging consensus for the structure of EmrE.Acta crystallographica. Section D, Biological crystallography · 2009Article
- Identification of a glycine motif required for packing in EmrE, a multidrug transporter from Escherichia coli.The Journal of biological chemistry · 2008Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
EmrE is a small H+-coupled multidrug transporter in Escherichia coli. Claims have been made for an antiparallel topology of this homodimeric protein. However, our own biochemical studies performed with detergent-solubilized purified protein support a parallel topology of the protomers. We developed an alternative approach to constrain the relative topology of the protomers within the dimer so that their activity can be assayed also in vivo before biochemical handling. Tandem EmrE was built with two identical monomers genetically fused tail to head (C-terminus of the first to N-terminus of the second monomer) with hydrophilic linkers of varying length. All the constructs conferred resistance to ethidium by actively removing it from the cytoplasm. The purified proteins bound substrate and transported methyl viologen into proteoliposomes by a proton-dependent mechanism. A tandem where one of the essential glutamates was replaced with glutamine transported only monovalent substrates and displayed a modified stoichiometry. The results support a parallel topology of the protomers in the functional dimer. The implications regarding insertion and evolution of membrane proteins are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.