ReviewPflugers Archiv : European journal of physiology2008
Pulling single molecules of titin by AFM--recent advances and physiological implications.
Review in Pflugers Archiv : European journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 121 citations in OpenAlex.
- Determination of Thermochemical Barriers in Multiple-Collision-Induced Dissociation Experiments on Gas-Phase Protein Complexes.Journal of the American Chemical Society · 2025Article
- Computed Vibrational Heat Capacities for Gas-Phase Biomolecular Ions.Journal of the American Society for Mass Spectrometry · 2025Article
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- Amyloidogenesis: What Do We Know So Far?International journal of molecular sciences · 2022Review
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- Measuring biological materials mechanics with atomic force microscopy - Mechanical unfolding of biopolymers.Microscopy research and technique · 2022Article
- Cell-Cell Mating Interactions: Overview and Potential of Single-Cell Force Spectroscopy.International journal of molecular sciences · 2022Review
- A multiscale sliding filament model of lymphatic muscle pumping.Biomechanics and modeling in mechanobiology · 2021Article
- Single-Molecule Force Spectroscopy Studies of Missense Titin Mutations That Are Likely Causing Cardiomyopathy.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Stretching single titin molecules from failing human hearts reveals titin's role in blunting cardiac kinetic reserve.Cardiovascular research · 2020Article
- Force generation by titin folding.Protein science : a publication of the Protein Society · 2017Article
- A HaloTag Anchored Ruler for Week-Long Studies of Protein Dynamics.Journal of the American Chemical Society · 2016Article
- Connecting thermal and mechanical protein (un)folding landscapes.Biophysical journal · 2014Article
- Probing muscle ankyrin-repeat protein (MARP) structure and function.Anatomical record (Hoboken, N.J. : 2007) · 2014Article
- Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels.Biophysical journal · 2014Article
- Mechano-signaling in heart failure.Pflugers Archiv : European journal of physiology · 2014Review
- Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.The Journal of cell biology · 2014Article
- Altered mechanical properties of titin immunoglobulin domain 27 in the presence of calcium.European biophysics journal : EBJ · 2013Article
- Phosphorylation modulates the mechanical stability of the cardiac myosin-binding protein C motif.Biophysical journal · 2013Article
- Mechanical unfolding studies of protein molecules.Biophysics (Nagoya-shi, Japan) · 2012Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Perturbation of a protein away from its native state by mechanical stress is a physiological process immanent to many cells. The mechanical stability and conformational diversity of proteins under force therefore are important parameters in nature. Molecular-level investigations of "mechanical proteins" have enjoyed major breakthroughs over the last decade, a development to which atomic force microscopy (AFM) force spectroscopy has been instrumental. The giant muscle protein titin continues to be a paradigm model in this field. In this paper, we review how single-molecule mechanical measurements of titin using AFM have served to elucidate key aspects of protein unfolding-refolding and mechanisms by which biomolecular elasticity is attained. We outline recent work combining protein engineering and AFM force spectroscopy to establish the mechanical behavior of titin domains using molecular "fingerprinting." Furthermore, we summarize AFM force-extension data demonstrating different mechanical stabilities of distinct molecular-spring elements in titin, compare AFM force-extension to novel force-ramp/force-clamp studies, and elaborate on exciting new results showing that AFM force clamp captures the unfolding and refolding trajectory of single mechanical proteins. Along the way, we discuss the physiological implications of the findings, not least with respect to muscle mechanics. These studies help us understand how proteins respond to forces in cells and how mechanosensing and mechanosignaling events may proceed in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.