ArticleBiology of reproduction2008
Regulation of murine lactate dehydrogenase C (Ldhc) gene expression.
Article in Biology of reproduction, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Cancer-testis antigen lactate dehydrogenase C4 as a novel biomarker of male infertility and cancer.Frontiers in oncology · 2022Review
- Correlation between promoter methylation of the LDH-C4 gene and DNMT expression in breast cancer and their prognostic significance.Oncology letters · 2022Article
- The Golgi Glycoprotein MGAT4D is an Intrinsic Protector of Testicular Germ Cells From Mild Heat Stress.Scientific reports · 2020Article
- Comparative expression profiling of testis-enriched genes regulated during the development of spermatogonial cells.PloS one · 2017Article
- In vivo microinjection and electroporation of mouse testis.Journal of visualized experiments : JoVE · 2014Article
- Human lactate dehydrogenase A (LDHA) rescues mouse Ldhc-null sperm function.Biology of reproduction · 2013Article
- Testis-specific lactate dehydrogenase is expressed in somatic tissues of plateau pikas.FEBS open bio · 2013Article
- Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.Reproduction (Cambridge, England) · 2013Article
- A-MYB (MYBL1) stimulates murine testis-specific Ldhc expression via the cAMP-responsive element (CRE) site.Biology of reproduction · 2012Article
- Distinct DNA methylation dynamics of spermatogenic cell-specific intronless genes is associated with CpG content.PloS one · 2012Article
- Functional cooperation between CREM and GCNF directs gene expression in haploid male germ cells.Nucleic acids research · 2010Article
- Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts.Biology of reproduction · 2008Article
- LDHC: the ultimate testis-specific gene.Journal of andrologyReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Expression of Ldhc begins with the onset of meiosis in male germ cells and continues throughout spermatogenesis. Transcriptional regulatory mechanisms, especially in primary spermatocytes, are poorly described because of the lack of a reliable cell culture system. We constructed mouse transgenics and transfected germ cells in situ to study expression of the testis-specific isozyme of lactate dehydrogenase (LDH). From previous work, we determined that a 100-bp Ldhc core promoter contained potential cis regulatory elements, including a palindrome (-21 to +10), GC box (-70 to -65), and cAMP-responsive element (CRE) sites (-53 to -49, -39 to -35). We provide here the demonstration of a functional role for these sequences by expression of mutated transgenes in vivo. Our results reveal for the first time that mutation of the GC box does not abolish promoter activity, which remains testis-specific. Mutation of GC box or CRE sites resulted in a 73% and 74% reduction in promoter activity, respectively, in a transient transfection of germ cells in vivo by electroporation; the combination of GC box and CRE site mutations eliminates promoter activity. Therefore, we conclude that simultaneous occupancy of the GC box and CRE sites in the core promoter is necessary for full expression of Ldhc in the testis.
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Registered trials
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