ArticleJournal of clinical microbiology2008
Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 195 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
195 citing papers in PubMed, 294 citations in OpenAlex.
- Trial
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- Virus detection and its association with symptoms during influenza-like illness in a sample of healthy adults enrolled in a randomised controlled vaccine trial.Influenza and other respiratory viruses · 2013Trial
- Sensitive detection and differentiation of rhinoviruses and enteroviruses by a nested real-time RT-PCR assay.Microbiology spectrum · 2026Article
- Molecular detection of respiratory pathogens in tonsillar tissue from asymptomatic children: implications for result interpretation among the asymptomatic and symptomatic.Microbiology spectrum · 2026Article
- Article
- Article
- Surveillance of respiratory viruses in severe acute respiratory infections in Southern Brazil, 2023-2024.BMC infectious diseases · 2025Article
- Epidemiology and genotypic diversity of rhinovirus in school-age children with acute respiratory illnesses seeking medical care.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2025Article
- Epigenomic and proteomic analyses provide insights into early-life immune regulation and asthma development in infants.Nature communications · 2025Article
- The differential burden of acute rhinovirus infections in children with underlying conditions.PloS one · 2025Article
- Zoonotic transmission of novel Influenza A variant viruses detected in Brazil during 2020 to 2023.Nature communications · 2024Article
- Article
- Laboratory validation of a clinical metagenomic next-generation sequencing assay for respiratory virus detection and discovery.Nature communications · 2024Article
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- Unveiling viral pathogens in acute respiratory disease: Insights from viral metagenomics in patients from the State of Alagoas, Brazil.PLoS neglected tropical diseases · 2024Article
- Integrated-omics analysis with explainable deep networks on pathobiology of infant bronchiolitis.NPJ systems biology and applications · 2024Article
- Characteristics of upper respiratory tract rhinovirus in children with allergic rhinitis and its role in disease severity.Microbiology spectrum · 2024Article
- Retrospective Genotyping of Enteroviruses Using a Diagnostic Nanopore Sequencing Workflow.Pathogens (Basel, Switzerland) · 2024Article
- Genetic Diversity and Detection of Respiratory Viruses Excluding SARS-CoV-2 during the COVID-19 Pandemic in Gabon, 2020-2021.Viruses · 2024Article
135 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human rhinoviruses (HRVs) are important contributors to respiratory disease, but their healthcare burden remains unclear, primarily because of the lack of sensitive, accurate, and convenient means of determining their causal role. To address this, we developed and clinically validated the sensitivity and specificity of a real-time reverse transcription-PCR (RT-PCR) assay targeting the viral 5' noncoding region defined by sequences obtained from all 100 currently recognized HRV prototype strains and 85 recently circulating field isolates. The assay successfully amplified all HRVs tested and could reproducibly detect 50 HRV RNA transcript copies, with a dynamic range of over 7 logs. In contrast, a quantified RNA transcript of human enterovirus 68 (HEV68) that showed the greatest sequence homology to the HRV primers and probe set was not detected below a concentration of 5 x 10(5) copies per reaction. Nucleic acid extracts of 111 coded respiratory specimens that were culture positive for HRV or HEV were tested with the HRV real-time RT-PCR assay and by two independent laboratories that used different in-house HRV/HEV RT-PCR assays. Eighty-seven HRV-culture-positive specimens were correctly identified by the real-time RT-PCR assay, and 4 of the 24 HEV-positive samples were positive for HRV. HRV-specific sequences subsequently were identified in these four specimens, suggesting HRV/HEV coinfection in these patients. The assay was successfully applied in an investigation of a coincidental outbreak of HRV respiratory illness among laboratory staff.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.