ArticleJournal of clinical microbiology2008
Development of a microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay for identification of clinically relevant human group A rotavirus G and P genotypes.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil.Revista do Instituto de Medicina Tropical de Sao Paulo · 2017Article
- Multiplexed one-step RT-PCR VP7 and VP4 genotyping assays for rotaviruses using updated primers.Journal of virological methods · 2015Article
- Analysis of human rotaviruses from a single location over an 18-year time span suggests that protein coadaption influences gene constellations.Journal of virology · 2014Article
- Molecular epidemiology of contemporary G2P[4] human rotaviruses cocirculating in a single U.S. community: footprints of a globally transitioning genotype.Journal of virology · 2014Article
- Application of PCR-ELISA in molecular diagnosis.BioMed research international · 2014Review
- Identification of novel Ghanaian G8P[6] human-bovine reassortant rotavirus strain by next generation sequencing.PloS one · 2014Article
- The global spread of rotavirus G10 strains: Detection in Ghanaian children hospitalized with diarrhea.The Journal of infectious diseases · 2010Article
- Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus strains with P[12] specificity.Virology journal · 2010Article
- Evolutionary dynamics of human rotaviruses: balancing reassortment with preferred genome constellations.PLoS pathogens · 2009Article
- Detection and genotyping of human rotavirus VP4 and VP7 genes by reverse transcriptase PCR and reverse hybridization.Journal of clinical microbiology · 2009Article
- Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab.Science (New York, N.Y.) · 2009Article
- Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus.Virology · 2009Article
- Group A rotavirus gastroenteritis: post-vaccine era, genotypes and zoonotic transmission.Einstein (Sao Paulo, Brazil)Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
A microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay (PCR-ELISA) has been used for the detection and identification of a variety of microorganisms. Here, we report the development of a PCR-ELISA for the identification of clinically relevant human rotavirus VP7 (G1 to G6, G8 to G10, and G12) and VP4 (P[4], P[6], P[8], P[9], and P[14]) genotypes. The G and P types of reference human and animal rotavirus strains for which specific probes were available were correctly identified by the PCR-ELISA. In addition, reference strains bearing G or P genotypes for which specific probes were unavailable, such as G11, G14, P[3], P[10], and P[11], did not display any cross-reactivity to the probes. The usefulness of the assay was further evaluated by analyzing a total of 396 rotavirus-positive stool samples collected in four countries: Brazil, Ghana, Japan, and the United States. The results of this study showed that the PCR-ELISA was sensitive and easy to perform without the use of any expensive and sophisticated equipment, the reagents used are easy to obtain commercially and advantageous over multiplex PCR since more than one type-specific probe is used and the selection of probes is more flexible.
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