ArticleAmerican journal of physiology. Endocrinology and metabolism2008
Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells.
Article in American journal of physiology. Endocrinology and metabolism, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Kruppel like factor 12 gene regulates ovarian granulosa cells proliferation and apoptosis via the PI3K/Akt signaling pathway.BMC veterinary research · 2026Article
- Luteinizing Hormone Surge-InducedBiomedicines · 2023Article
- Review
- Bisphenol A and bisphenol S both disrupt ovine granulosa cell steroidogenesis but through different molecular pathways.Journal of ovarian research · 2023Article
- The study of candidate genes in the improvement of egg production in ducks - a review.Poultry science · 2022Review
- Transcriptome-wide N6-methyladenine methylation in granulosa cells of women with decreased ovarian reserve.BMC genomics · 2022Article
- NRF2-mediated signaling is a master regulator of transcription factors in bovine granulosa cells under oxidative stress condition.Cell and tissue research · 2021Article
- Deletion of Krüppel-like factor-4 promotes axonal regeneration in mammals.Neural regeneration research · 2021Article
- Transcriptome Analysis of the Chicken Follicular Theca Cells with miR-135a-5p Suppressed.G3 (Bethesda, Md.) · 2020Article
- LH-induced Transcriptional Regulation ofInternational journal of molecular sciences · 2020Article
- Role ofInternational journal of molecular sciences · 2018Article
- Erythropoietin-producing hepatocellular A7 triggering ovulation indicates a potential beneficial role for polycystic ovary syndrome.EBioMedicine · 2018Article
- The Krüppel-like factors in female reproductive system pathologies.Journal of molecular endocrinology · 2015Review
- Article
- The reproductive phenotype of mice null for transcription factor Krüppel-like factor 13 suggests compensatory function of family member Krüppel-like factor 9 in the peri-implantation uterus.Biology of reproduction · 2012Article
- Oocyte-somatic cells interactions, lessons from evolution.BMC genomics · 2012Article
- Article
- WNT4 is required for normal ovarian follicle development and female fertility.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Krüppel-like factors 4 and 5: unity in diversity.Current genomics · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Kruppel-like factors (KLFs) are important Sp1-like eukaryotic transcriptional proteins. The LDLR, StAR, and CYP11A genes exhibit GC-rich Sp1-like sites, which have the potential to bind KLFs in multiprotein complexes. We now report that KLF4, KLF9, and KLF13 transcripts are expressed in and regulate ovarian cells. KLF4 and 13, but not KLF9, mRNA expression was induced and then repressed over time (P < 0.001). Combined LH and IGF-I stimulation increased KLF4 mRNA at 2 h (P < 0.01), whereas LH decreased KLF13 mRNA at 6 h (P < 0.05), and IGF-I reduced KLF13 at 24 h (P < 0.01) compared with untreated control. KLF9 was not regulated by either hormone. Transient transfection of KLF4, KLF9, and KLF13 suppressed LDLR/luc, StAR/luc, and CYP11A/luc by 80-90% (P < 0.001). Histone-deacetylase (HDAC) inhibitors stimulated LDLR/luc five- to sixfold and StAR/luc and CYP11A/luc activity twofold (P < 0.001) and partially reversed suppression by all three KLFs (P < 0.001). Deletion of the zinc finger domain of KLF13 abrogated repression of LDLR/luc. Lentiviral overexpression of the KLF13 gene suppressed LDLR mRNA (P < 0.001) and CYP11A mRNA (P = 0.003) but increased StAR mRNA (P = 0.007). Collectively, these data suggest that KLFs may recruit inhibitory complexes containing HDAC corepressors, thereby repressing LDLR and CYP11A transcription. Conversely, KLF13 may recruit unknown coactivators or stabilize StAR mRNA, thereby explaining enhancement of in situ StAR gene expression. These data introduce new potent gonadal transregulators of genes encoding proteins that mediate sterol uptake and steroid biosynthesis.
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