Evidence map›Paper›PMID 18056791›Full record

Trial reportAmerican journal of physiology. Endocrinology and metabolism2008

Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle.

Hans C Dreyer, Micah J Drummond, Bart Pennings, Satoshi Fujita, Erin L Glynn, David L Chinkes, Shaheen Dhanani, Elena Volpi, Blake B Rasmussen

2 registry-linked trialsOpen access · greenAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in American journal of physiology. Endocrinology and metabolism, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 194 papers, 8 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
194citing papers in PubMed, 8 pooled it
7.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT00760383 nacompletednot on this map

Muscle Regrowth During Physical Rehabilitation and Amino Acid Supplementation

TypeinterventionalSponsorUniversity of OregonRan2008 to 2013Enrolled60ConditionsOsteoarthritisArmsEssential amino acids, Alanine
NCT00891696 phase1completednot on this mapstarted 2009, after this paper: background citation

Nutritional and Contractile Regulation of Muscle Growth (Cycle 2)

TypeinterventionalSponsorThe University of Texas Medical Branch, GalvestonRan2009 to 2015Enrolled144ConditionsSarcopeniaArmsRapamycin, Amino acid supplementation, Low-intensity resistance exercise, Sodium nitroprusside, Blood flow restriction cuff
3 · Its place in the literature

Who cites it

194 citing papers in PubMed, 8 syntheses or guidelines pooled it, 439 citations in OpenAlex.

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134 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Hans C DreyerDepartment of Physical Therapy, Division of Rehabilitation Sciences, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-1144, USA.
Micah J Drummond
Bart Pennings
Satoshi Fujita
Erin L Glynn
David L Chinkes
Shaheen Dhanani
Elena Volpi
Blake B Rasmussen
The University of Texas Medical Branch at Galveston · US

Funding

ZOLLINGER-ELLISON SYNDROME AND DUODONAL ULCER DISEASEM01RR000073 · NCRR · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI ANDERSON, KARL ELMO · 1985 to 2009
$31.3M
UTMB OAIC Research Education Component (REC)P30AG024832 · NIA · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI JAMES S. GOODWIN, MD, MELISSA M. MORROW · 2005 to 2026
$26.7M
Nutritional and Contractile Regulation of Muscle GrowthR01AR049877 · NIAMS · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI RASMUSSEN, BLAKE B · 2004 to 2012
$3.3M
Interdisciplinary Rehabilitation Research TrainingT32HD007539 · NICHD · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI OTTENBACHER, KENNETH J. · 2001 to 2011
$1.1M
Gas Chromatograph Mass Spectrometer with Combined FIDS10RR016650 · NCRR · UNIVERSITY OF SOUTHERN CALIFORNIA · PI VOLPI, ELENA · 2002 to 2002
$146k
NCRR NIH HHS M01 RR000073NCRR NIH HHS M01 RR 00073NCRR NIH HHS S10 RR016650NCRR NIH HHS S10 RR 16650NIAMS NIH HHS R01 AR 049877NIAMS NIH HHS R01 AR049877NIA NIH HHS P30 AG 024832NIA NIH HHS P30 AG024832NICHD NIH HHS T32 HD007539PHS HHS H133 PO 40003
6 · The paper itself

Abstract

We recently showed that resistance exercise and ingestion of essential amino acids with carbohydrate (EAA+CHO) can independently stimulate mammalian target of rapamycin (mTOR) signaling and muscle protein synthesis in humans. Providing an EAA+CHO solution postexercise can further increase muscle protein synthesis. Therefore, we hypothesized that enhanced mTOR signaling might be responsible for the greater muscle protein synthesis when leucine-enriched EAA+CHOs are ingested during postexercise recovery. Sixteen male subjects were randomized to one of two groups (control or EAA+CHO). The EAA+CHO group ingested the nutrient solution 1 h after resistance exercise. mTOR signaling was assessed by immunoblotting from repeated muscle biopsy samples. Mixed muscle fractional synthetic rate (FSR) was measured using stable isotope techniques. Muscle protein synthesis and 4E-BP1 phosphorylation during exercise were significantly reduced (P < 0.05). Postexercise FSR was elevated above baseline in both groups at 1 h but was even further elevated in the EAA+CHO group at 2 h postexercise (P < 0.05). Increased FSR was associated with enhanced phosphorylation of mTOR and S6K1 (P < 0.05). Akt phosphorylation was elevated at 1 h and returned to baseline by 2 h in the control group, but it remained elevated in the EAA+CHO group (P < 0.05). 4E-BP1 phosphorylation returned to baseline during recovery in control but became elevated when EAA+CHO was ingested (P < 0.05). eEF2 phosphorylation decreased at 1 and 2 h postexercise to a similar extent in both groups (P < 0.05). Our data suggest that enhanced activation of the mTOR signaling pathway is playing a role in the greater synthesis of muscle proteins when resistance exercise is followed by EAA+CHO ingestion.

Indexed as

Absorptiometry, PhotonAdultAmino Acids, EssentialBlotting, WesternCross-Sectional StudiesDietary CarbohydratesElectrophoresis, Polyacrylamide GelExerciseGlucoseHumansLactic AcidLeucineMaleMuscle ProteinsMuscle, SkeletalOncogene Protein v-aktAmino Acids, EssentialDietary CarbohydratesGlucoseLactic AcidLeucineMTOR protein, humanMuscle ProteinsOncogene Protein v-aktPhenylalanineProtein KinasesRibosomal Protein S6 KinasesTOR Serine-Threonine KinasesTuberous Sclerosis Complex 2 ProteinTumor Suppressor Proteins

Identifiers

PMID18056791
PMCPMC2706121
OpenAlexW2135999406

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.