ArticleNucleic acids research2008
Regulating gene expression in human leukemia cells using light-activated oligodeoxynucleotides.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 84 citations in OpenAlex.
- Development of label-free light-controlled gene expression technologies using mid-IR and terahertz light.Frontiers in bioengineering and biotechnology · 2024Review
- Sequence-independent, site-specific incorporation of chemical modifications to generate light-activated plasmids.Chemical science · 2023Article
- Quantitative analysis of miRNAs using SplintR ligase-mediated ligation of complementary-pairing probes enhanced by RNase H (SPLICER)-qPCR.Molecular therapy. Nucleic acids · 2023Article
- Expanding Catch and Release DNA Decoy (CRDD) Technology with Pyrimidine Mimics.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Translational control of gene function through optically regulated nucleic acids.Chemical Society reviews · 2021Review
- Photoactivatable Circular Caged Oligonucleotides for TranscriptomeChemPhotoChem · 2021Article
- Conditionally Activated ("Caged") Oligonucleotides.Molecules (Basel, Switzerland) · 2021Review
- Controlling gene expression with light: a multidisciplinary endeavour.Biochemical Society transactions · 2020Review
- Multiplexed Photoactivation of mRNA with Single-Cell Resolution.ACS chemical biology · 2020Article
- Optochemical Control of Biological Processes in Cells and Animals.Angewandte Chemie (International ed. in English) · 2018Review
- Light-Activated Control of Translation by Enzymatic Covalent mRNA Labeling.Angewandte Chemie (International ed. in English) · 2018Article
- A study on endonuclease BspD6I and its stimulus-responsive switching by modified oligonucleotides.PloS one · 2018Article
- Stimuli-responsive oligonucleotides in prodrug-based approaches for gene silencing.Beilstein journal of organic chemistry · 2018Review
- Caged oligonucleotides for studying biological systems.Journal of inorganic biochemistry · 2015Review
- Light-mediated activation of siRNA Release in diblock copolymer assemblies for controlled gene silencing.Advanced healthcare materials · 2015Article
- Site-specific promoter caging enables optochemical gene activation in cells and animals.Journal of the American Chemical Society · 2014Article
- Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach.Accounts of chemical research · 2014Article
- Optochemical control of RNA interference in mammalian cells.Nucleic acids research · 2013Article
- Cellular delivery and photochemical activation of antisense agents through a nucleobase caging strategy.ACS chemical biology · 2013Article
- Caged oligonucleotides for bidirectional photomodulation of let-7 miRNA in zebrafish embryos.Bioorganic & medicinal chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Light-activated antisense oligodeoxynucleotides (asODNs) were developed to control the degradation of target mRNA in living cells by RNase H. A 20-mer asODN previously shown to target c-myb, a hematopoietic transcription factor, was covalently attached via a photocleavable linker (PL) to partially complementary 20-mer sense strands (sODNs). In the 'caged' state, the sODN blocked hybridization of the asODN to c-myb mRNA. Six asODN-PL-sODN conjugates, C1-C6, were synthesized. C5, with twelve complementary bases, gave the largest decrease in melting temperature (T(m)) upon UV irradiation (DeltaT(m) = -29 degrees C). The most thermally stable conjugate, C6 (T(m) = 84 degrees C), gave the lowest background RNase H activity, with just 8.6% degradation of an RNA 40-mer after 1 h incubation. In biochemical assays with C6, RNA digestion increased 10-fold 10 min after UV irradiation. Finally, phosphorothioated analogs S-C5 and S-C6 were synthesized to test activity in cultured K562 (human leukemia) cells. No knockdown of c-myb mRNA or protein was observed with intact S-C5 or S-C6, whereas more than half of c-myb mRNA was degraded 24 h after photoactivation. Two-fold photomodulation of c-MYB protein levels was also observed with S-C5. However, no photomodulation of c-MYB protein levels was observed with S-C6, perhaps due to the greater stability of this duplex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.