ArticleThe Plant cell2007
Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 69 citations in OpenAlex.
- Membranous translation platforms in the chloroplast of Chlamydomonas reinhardtii.Plant physiology · 2025Article
- Assembly and Repair of Photosystem II inPlants (Basel, Switzerland) · 2024Review
- The availability of neither D2 nor CP43 limits the biogenesis of photosystem II in tobacco.Plant physiology · 2021Article
- Pas de Trois: An Overview of Penta-, Tetra-, and Octo-Tricopeptide Repeat Proteins FromFrontiers in plant science · 2021Review
- The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo inThe Plant cell · 2020Article
- Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas.The Plant cell · 2019Article
- An RNA Chaperone-Like Protein Plays Critical Roles in Chloroplast mRNA Stability and Translation in Arabidopsis and Maize.The Plant cell · 2019Article
- Article
- Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.Nucleic acids research · 2017Article
- Dynamics of Chloroplast Translation during Chloroplast Differentiation in Maize.PLoS genetics · 2016Article
- A nucleus-encoded chloroplast protein regulated by iron availability governs expression of the photosystem I subunit PsaA in Chlamydomonas reinhardtii.Plant physiology · 2015Article
- Redox regulation of thylakoid protein kinases and photosynthetic gene expression.Antioxidants & redox signaling · 2013Review
- How to build functional thylakoid membranes: from plastid transcription to protein complex assembly.Planta · 2013Review
- Reciprocal regulation of protein synthesis and carbon metabolism for thylakoid membrane biogenesis.PLoS biology · 2013Article
- The atypical short-chain dehydrogenases HCF173 and HCF244 are jointly involved in translational initiation of the psbA mRNA of Arabidopsis.Plant physiology · 2012Article
- Biogenic membranes of the chloroplast in Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Quantitative shotgun proteomics using a uniform ¹⁵N-labeled standard to monitor proteome dynamics in time course experiments reveals new insights into the heat stress response of Chlamydomonas reinhardtii.Molecular & cellular proteomics : MCP · 2011Article
- Messenger RNA degradation is initiated at the 5' end and follows sequence- and condition-dependent modes in chloroplasts.Nucleic acids research · 2011Article
- Manipulating RuBisCO accumulation in the green alga, Chlamydomonas reinhardtii.Plant molecular biology · 2011Article
- Expression of plastid genes: organelle-specific elaborations on a prokaryotic scaffold.Plant physiology · 2011Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression in chloroplasts is regulated mainly at the posttranscriptional level. In the green alga Chlamydomonas reinhardtii, synthesis of the D2 protein (PsbD), which is the rate-determining subunit for the assembly of photosystem II, depends on the RNA stability factor Nac2. In addition, the RNA binding protein RBP40 has been implicated in translational control via a U-rich element in the 5' untranslated region (5'UTR) of the psbD mRNA. Here, we report the identification of the RBP40 gene based on mass spectrometric analysis of its purified product. Unexpectedly, this was found to be identical to the previously described RNA binding protein RB38, which had been suggested to be involved in the regulation of D1 protein synthesis. However, we show that RBP40 binds to the psbD 5'UTR in a Nac2-dependent fashion both in vitro and in vivo. Molecular characterization of RBP40 RNA interference lines confirmed that RBP40 specifically affects the initiation of D2 synthesis. Native polyacrylamide gel electrophoresis, coimmunoprecipitation, and sedimentation analyses revealed that Nac2 and RBP40 form parts of a complex of 550 kD that is displaced from the psbD mRNA prior to polysome assembly. Together, these data indicate that the processes of 5'UTR-mediated RNA stabilization and translation initiation are tightly coupled in Chlamydomonas.
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