ArticleBiophysical journal2008
Measuring and modeling chloride-hydroxyl exchange in the Guinea-pig ventricular myocyte.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Acidic environments trigger intracellular H+-sensing FAK proteins to re-balance sarcolemmal acid-base transporters and auto-regulate cardiomyocyte pH.Cardiovascular research · 2022Article
- Protocol to record and quantify the intracellular pH in contracting cardiomyocytes.STAR protocols · 2022Article
- Article
- Construction of a High-Density Genetic Map and Identification of Quantitative Trait Loci for Nitrite Tolerance in the Pacific White Shrimp (Frontiers in genetics · 2020Article
- Action Potential Shortening and Impairment of Cardiac Function by Ablation ofCirculation. Arrhythmia and electrophysiology · 2017Article
- Functional interaction between bicarbonate transporters and carbonic anhydrase modulates lactate uptake into mouse cardiomyocytes.Pflugers Archiv : European journal of physiology · 2015Article
- Critical role of bicarbonate and bicarbonate transporters in cardiac function.World journal of biological chemistry · 2014Review
- Resistance to cardiomyocyte hypertrophy in ae3-/- mice, deficient in the AE3 Cl-/HCO3- exchanger.BMC cardiovascular disorders · 2014Article
- The chemistry, physiology and pathology of pH in cancer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Human-induced pluripotent stem cell-derived cardiomyocytes for studies of cardiac ion transporters.American journal of physiology. Cell physiology · 2013Article
- Regulation of ion gradients across myocardial ischemic border zones: a biophysical modelling analysis.PloS one · 2013Article
- Effect of nitric oxide donors S-nitroso-N-acetyl-DL-penicillamine, spermine NONOate and propylamine propylamine NONOate on intracellular pH in cardiomyocytes.Clinical and experimental pharmacology & physiology · 2012Article
- Loss of the AE3 anion exchanger in a hypertrophic cardiomyopathy model causes rapid decompensation and heart failure.Journal of molecular and cellular cardiology · 2011Article
- New insights into the physiological role of carbonic anhydrase IX in tumour pH regulation.Oncogene · 2010Review
- A model of Na+/H+ exchanger and its central role in regulation of pH and Na+ in cardiac myocytes.Biophysical journal · 2009Article
- Putative re-entrant loop 1 of AE2 transmembrane domain has a major role in acute regulation of anion exchange by pH.The Journal of biological chemistry · 2009Article
- Ion transport in the intestine.Current opinion in gastroenterology · 2009Review
- The SLC26 gene family of anion transporters and channels.Molecular aspects of medicineReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Protons are powerful modulators of cardiac function. Their intracellular concentration is regulated by sarcolemmal ion transporters that export or import H+-ions (or their ionic equivalent: HCO3-, OH-). One such transporter, which imports H+-equivalents, is a putative Cl-/OH- exchanger (CHE). A strong candidate for CHE is SLC26A6 protein, a product of the SLC26A gene family of anion transporters, which has been detected in murine heart. SLC26A6 protein is suggested to be an electrogenic 1Cl-/2OH-(2HCO3-) exchanger. Unfortunately, there is insufficient characterization of cardiac CHE against which the properties of heterologously expressed SLC26A6 can be matched. We therefore investigated the proton, Cl-, and voltage dependence of CHE activity in guinea-pig ventricular myocytes, using voltage-clamp, intracellular pH fluorescence, and mathematical modeling techniques. We find that CHE activity is tightly regulated by intracellular and extracellular pH, is voltage-insensitive over a wide range (+/-80 mV), and displays substrate dependence suggestive of electroneutral 1Cl-/1OH- exchange. These properties exclude electrogenic SLC26A6 as sole contributor to CHE. Either the SLC26A6 product in heart is electroneutral, or CHE comprises at least two transporters with oppositely balanced voltage sensitivity. Alternatively, CHE may comprise an H+-Cl- coinflux system, which cannot be distinguished kinetically from an exchanger. Irrespective of ionic mechanism, CHE's pH sensitivity helps to define resting intracellular pH, and hence basal function in the heart.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.