ArticleStem cells (Dayton, Ohio)2008
Fluorophore-conjugated iron oxide nanoparticle labeling and analysis of engrafting human hematopoietic stem cells.
Article in Stem cells (Dayton, Ohio), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- Applications of Gene Editing and Nanotechnology in Stem Cell-Based Therapies for Human Diseases.Stem cell reviews and reports · 2025Review
- Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation.Pharmaceutics · 2023Article
- Nanoparticles targeting hematopoietic stem and progenitor cells: Multimodal carriers for the treatment of hematological diseases.Frontiers in genome editing · 2022Review
- Introducing the Tellurophene-Appended BODIPY: PDT Agent with Mass Cytometry Tracking Capabilities.ACS medicinal chemistry letters · 2021Article
- Studies on Aggregated Nanoparticles Steering during Deep Brain Membrane Crossing.Nanomaterials (Basel, Switzerland) · 2021Article
- Article
- Stem cell homing, tracking and therapeutic efficiency evaluation for stroke treatment using nanoparticles: A systematic review.World journal of stem cells · 2020Article
- Transient loading of CD34International journal of nanomedicine · 2017Article
- Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation.Beilstein journal of nanotechnology · 2015Article
- Labeling human embryonic stem-cell-derived cardiomyocytes for tracking with MR imaging.Pediatric radiology · 2011Article
- In situ labeling and magnetic resonance imaging of transplanted human hepatic stem cells.Molecular imaging and biology · 2011Article
- Characterization and in vivo testing of mesenchymal stem cells derived from human embryonic stem cells.Tissue engineering. Part A · 2011Article
- Gd₂O₃ nanoparticles in hematopoietic cells for MRI contrast enhancement.International journal of nanomedicine · 2011Article
- Longitudinal tracking of human dendritic cells in murine models using magnetic resonance imaging.Magnetic resonance in medicine · 2010Article
- Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging.Journal of nanobiotechnology · 2010Article
- Non-invasive tracking of human haemopoietic CD34(+) stem cells in vivo in immunodeficient mice by using magnetic resonance imaging.European radiology · 2010Article
- Multimodality imaging probes: design and challenges.Chemical reviews · 2010Review
- Human cord blood progenitors with high aldehyde dehydrogenase activity improve vascular density in a model of acute myocardial infarction.Journal of translational medicine · 2010Article
- A simple and highly sensitive method for magnetic nanoparticle quantitation using 1H-NMR spectroscopy.Biophysical journal · 2009Article
- Enhanced stem cell tracking via electrostatically assembled fluorescent SPION-peptide complexes.Nanotechnology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
The use of nanometer-sized iron oxide particles combined with molecular imaging techniques enables dynamic studies of homing and trafficking of human hematopoietic stem cells (HSC). Identifying clinically applicable strategies for loading nanoparticles into primitive HSC requires strictly defined culture conditions to maintain viability without inducing terminal differentiation. In the current study, fluorescent molecules were covalently linked to dextran-coated iron oxide nanoparticles (Feridex) to characterize human HSC labeling to monitor the engraftment process. Conjugating fluorophores to the dextran coat for fluorescence-activated cell sorting purification eliminated spurious signals from nonsequestered nanoparticle contaminants. A short-term defined incubation strategy was developed that allowed efficient labeling of both quiescent and cycling HSC, with no discernable toxicity in vitro or in vivo. Transplantation of purified primary human cord blood lineage-depleted and CD34(+) cells into immunodeficient mice allowed detection of labeled human HSC in the recipient bones. Flow cytometry was used to precisely quantitate the cell populations that had sequestered the nanoparticles and to follow their fate post-transplantation. Flow cytometry endpoint analysis confirmed the presence of nanoparticle-labeled human stem cells in the marrow. The use of fluorophore-labeled iron oxide nanoparticles for fluorescence imaging in combination with flow cytometry allows evaluation of labeling efficiencies and homing capabilities of defined human HSC subsets.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.